African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6801

Full Length Research Paper

Effect of pH on growth of the mycelium of Trichoderma viride and Pleurotus ostreatus in solid cultivation mediums

Omar Romero-Arenas1*, Miguel Ángel Damián Huato1, Israel Hernández Treviño2, J. F. C. Parraguire Lezama2, Agustín Aragón García1 and Alfonso Daniel Victoria Arellano2    
1Center for Agroecology (CENAGRO-BUAP), Mexico. 2Agroforestry Engineering School, Campus Tetela BUAP, Mexico.
Email: [email protected]

  •  Accepted: 06 June 2012
  •  Published: 04 September 2012


In the cultivation of oyster mushroom (Pleurotus spp), various contaminants moulds such as Trichoderma spp that are often found in production plants, leading to losses exceeding 77% in a short periods of production were identified. This research studied the effect of pH on two solid cultivation mediums, potato dextrose agar (PDA) and yeast complete medium (YCM), evaluating mycelial growth and the rate of development of strains of Trichoderma viride and Pleurotus ostreatus. The pH levels studied were 7, 9 and 11 approximately which were obtained with sodium hydroxide (NaOH-1N), Ca(OH)2 and CaS04 (lime and gypsum commercial); the control group has a pH of 5.7 according to the manufacturer. The strain of P. ostreatus (CP-50) obtains a development rate of 6.10 mm / day at pH 11.2 presenting tolerance at alkaline pH; however, the strain of T. viride (CP-T4) has a rate of development of 0.41 mm / day at pH 11.2 showing negative effects on growth and development in alkaline pH.


Key words: Trichoderma spp., Pleurotus spp., pH, development rate, mycelial growth.