African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6691

Full Length Research Paper

Physiological and biochemical analysis of Trichoderma species isolated from different location U. P.

Anuradha Singh, Sonika Pandey*
  • Anuradha Singh, Sonika Pandey*
  • Biocontrol Laboratory, Department of Plant Pathology, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur-208002, Uttar Pradesh, India.
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Mukesh Srivastava
  • Mukesh Srivastava
  • Biocontrol Laboratory, Department of Plant Pathology, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur-208002, Uttar Pradesh, India.
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Mohammad Shahid
  • Mohammad Shahid
  • Biocontrol Laboratory, Department of Plant Pathology, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur-208002, Uttar Pradesh, India.
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Vipul Kumar
  • Vipul Kumar
  • Biocontrol Laboratory, Department of Plant Pathology, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur-208002, Uttar Pradesh, India.
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Shubha Trivadi
  • Shubha Trivadi
  • Biocontrol Laboratory, Department of Plant Pathology, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur-208002, Uttar Pradesh, India.
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Ved Ratan
  • Ved Ratan
  • Biocontrol Laboratory, Department of Plant Pathology, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur-208002, Uttar Pradesh, India.
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  •  Received: 01 September 2015
  •  Accepted: 26 September 2015
  •  Published: 31 December 2015

Abstract

Seven different species of Trichoderma isolated from the different locations of Uttar Pradesh were used in this study. A comparative analysis was done among all the seven species for the determination of glucanase enzyme production and nitrogen content determination the results showed that among all the seven tested species, Trichoderma harzianum shows the highest glucanase enzyme production as well as the highest nitrogen content. Optimal physical parameters such as pH, temperature, and effect of agitation were also tested for the biomass production on all the eight species. The results revealed that the optimal pH temp and agitation speed for Trichoderma biomass production were 6.0 and 6.5, 25 and 30°C and 150 rpm. In present study we also evaluated the effect of two different carbon sources on the glucanase enzyme induction. Carboxymethyl cellulose (CMC) was found to be the best carbon source for glucanase enzyme induction.
 
Key words: Trichoderma, nitrogen content, biological control.