African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6691

Full Length Research Paper

Construction of a proteomic reference map for analysis of development of etiolated rice seedlings

Bin Wang, Lequn Kang, Hengchuan Xia, Keming Zhu, Min Guo, Xiaoyong Liu, Qin Yao and Keping Chen*
Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang, Jiangsu 212013, P. R. China.
Email: [email protected]

  •  Accepted: 12 January 2012
  •  Published: 29 February 2012

Abstract

 

When rice seedlings grow in darkness, they will be yellow in color and show typical etiolated characters such as elongation of hypocotyls and stem, rolling up of young leaves around the stem tip. To better understand the development of etiolated rice seedlings at the molecule level, a proteomic reference map of etiolated rice seedlings was established by combining Two-Dimensional Gel Electrophoresis (2-DE) and matrix-assisted laser desorption/ionization–time-of-flight-mass spectrometry (MALDI-TOF-MS). In this study, a total of 1170 protein spots were detected. 200 protein spots were picked for MALDI-TOF-MS analysis, and 162 proteins were chosen for further analysis and construction of the proteomic reference map. As expected, most of the chosen proteins were metabolism and energy metabolism-associated, suggesting that the early stages of development of etiolated rice seedlings involved in complex metabolic activities. We further discussed the potential functions of these proteins in the development of etiolated rice seedlings. Our data also showed that a large number of proteins had not yet been annotated in rice.

 

Key words: Etiolated rice seedlings, proteomic, two-dimensional gel electrophoresis, matrix-assisted laser desorption/ionization–time-of-flight-mass spectrometry.