African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6691

Full Length Research Paper

Genetic variability of Fusarium solani and Fusarium oxysporum f. sp. passiflorae isolates from Pantanal, Amazon and Cerrado biomes of Mato Grosso, Brazil

Arielen Barreto de Carvalho
  • Arielen Barreto de Carvalho
  • Universidade do Estado de Mato Grosso (UNEMAT), Cáceres, MT, Brazil.
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Valdomiro José Coelho
  • Valdomiro José Coelho
  • Universidade do Estado de Mato Grosso (UNEMAT), Cáceres, MT, Brazil.
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Kelly Lana Araújo
  • Kelly Lana Araújo
  • Universidade do Estado de Mato Grosso (UNEMAT), Cáceres, MT, Brazil.
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Kátia Aparecida de Siqueira
  • Kátia Aparecida de Siqueira
  • Universidade Federal de Mato Grosso (UFMT), Cuiabá, MT, Brazil.
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Sandra Mara Alves da Silva Neves
  • Sandra Mara Alves da Silva Neves
  • Universidade do Estado de Mato Grosso (UNEMAT), Cáceres, MT, Brazil.
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Marcos Antônio Soares
  • Marcos Antônio Soares
  • Universidade Federal de Mato Grosso (UFMT), Cuiabá, MT, Brazil.
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Leonarda Grillo Neves
  • Leonarda Grillo Neves
  • Universidade do Estado de Mato Grosso (UNEMAT), Cáceres, MT, Brazil.
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  •  Received: 26 August 2015
  •  Accepted: 03 November 2015
  •  Published: 31 December 2015

Abstract

Soil fungi, Fusarium solani and Fusarium oxysporum f. sp. passiflorae are among the pathogens of greatest economic importance to the passion fruit culture. Little is known about the genetic variability of these pathogens from different regions. The genetic variability of F. solani and F. oxysporum f. sp. passiflorae isolates from Pantanal, Amazon and Cerrado of Mato Grosso State, Brazil, was studied with the help of molecular markers inter-simple sequence repeat amplification (ISSR) and inter-retrotransposon amplified polymorphism (IRAP). The molecular identification of the isolates was performed by partial sequencing of the internal transcribed spacer (ITS) of the rDNA region. High genetic variability among isolates of F. solani and F. oxysporum f. sp. passiflorae was found. The dendrogram generated by UPGMA divided the fungi isolates in two large groups, however, with grouping tendency according to the biome of collection only for F. oxysporum f. sp. passiflorae. There was no significant correlation between geographic distance and genetic similarity for F. oxysporum f. sp. passiflorae and F. solani.

Key words: Collar rot, Fusarium wilt, passionflower, Passiflora edulis, inter-simple sequence repeat amplification (ISSR), inter-retrotransposon amplified polymorphism (IRAP).