African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6688

Full Length Research Paper

High performance liquid chromatography-diode array detector (HPLC-DAD) detection of trans-resveratrol: Evolution during ripening in grape berry skins

Angelo Maria Giuffrè
  • Angelo Maria Giuffrè
  • Università degli Studi Mediterranea di Reggio Calabria – Dipartimento di Agraria.
  • Google Scholar

  •  Accepted: 03 January 2013
  •  Published: 31 January 2013



Trans-resveratrol (3, 5, 4' – trihydroxy-trans-stilbene) is a stilbene naturally present in a number of plant families. Berry skins of grape (Vitis vinifera L.), Alicante, Black Malvasia, Nerello and Prunesta cultivars were analyzed at weekly intervals for trans-resveratrol production at five ripening stages during the last five weeks of maturation, from August 26th to September 23th. Analysis was carried out using the high performance liquid chromatography-diode array detector (HPLC-DAD) technique after fractioning of trans-resveratrol through a 500 mg C18 column (Solid Phase Isolation – SPI technique). A continuous decrease in trans-resveratrol content in all cultivars was observed during ripening. Alicante had the highest trans-resveratrol content, showing a decrease of 46% from the first to last sampling, while Black Malvasia produced the lowest amount of trans-resveratrol, 4 to 5 times less than Alicante at each sampling date.


Key words: Antioxidants, grape, phenols, stilbenes, trans-resveratrol, high performance liquid chromatograph (HPLC).