African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6688

Full Length Research Paper

Genetic divergence of Dalbergia sissoo through random amplified polymorphic DNA analysis at different districts of Punjab Pakistan

Tanveer Hussain1*, Ghulam Sarwar Khan2, Shahid Ali Khan3 and Muhammad Ashfaq4 and Nasir Masood1
  1College of Agriculture and Environmental Sciences, Islamia University of Bahawalpur, Pakistan. 2Department of Forestry, Range Management and Wildlife University of Agriculture, Faisalabad, Pakistan. 3Department of Research and Planning, Arid Agriculture University, Rawalpindi, Pakistan. 4Department of Chemistry, Islamia University of Bahawalpur, Pakistan.
Email: [email protected]

  •  Accepted: 05 January 2012
  •  Published: 29 February 2012

Abstract

 

Genetic diversity of eight trees of Dalbergia germplasm as collected from three different ecological zones of the Punjab (Lahore, Bahawalpur and Faisalabad). All samples were characterized through random amplified polymorphic DNA (RAPD) with agarose gel electrophoreses techniques. The 25 primers screened out from 50 primers based on their polymorphic nature. Total numbers of 182 polymorphic fragments obtained and 35 and 14 polymorphic fragments selected in the Dalbergia healthy dark green and diseased yellow plants respectively. The healthy samples differentiated from diseased samples on the bases of their DNA base pairs and bands size. The healthy samples showed strong and bright bands 4200 bp with 25 ng and 500 bp with 50 ng DNA quantities. While diseased samples showed weak/faint bands at 4200 bp with 3 ng and 500 bp is only 8 ng quantities. The range of similarity matrix was 68 to 88% according to the DNA polymorphic loci estimated by Nei’s similarity index. Trees of Bahawalpur and Lahore zone have least 68% genetic similarity. The trees from Faisalabad and Bahawalpur have 88% genetic similarity. Diseased trees from Lahore and Bahawalpur zones have 68% genetic similarity.

 

Key words: Agarose gel electrophoresis, Delbergia sissoo L., Genetics analyisis, RAPD.