Abstract

Genetic diversity of eight trees of Dalbergia germplasm as collected from three different ecological zones of the Punjab (Lahore, Bahawalpur and Faisalabad). All samples were characterized through random amplified polymorphic DNA (RAPD) with agarose gel electrophoreses techniques. The 25 primers screened out from 50 primers based on their polymorphic nature. Total numbers of 182 polymorphic fragments obtained and 35 and 14 polymorphic fragments selected in the Dalbergia healthy dark green and diseased yellow plants respectively. The healthy samples differentiated from diseased samples on the bases of their DNA base pairs and bands size. The healthy samples showed strong and bright bands 4200 bp with 25 ng and 500 bp with 50 ng DNA quantities. While diseased samples showed weak/faint bands at 4200 bp with 3 ng and 500 bp is only 8 ng quantities. The range of similarity matrix was 68 to 88% according to the DNA polymorphic loci estimated by Nei’s similarity index. Trees of Bahawalpur and Lahore zone have least 68% genetic similarity. The trees from Faisalabad and Bahawalpur have 88% genetic similarity. Diseased trees from Lahore and Bahawalpur zones have 68% genetic similarity.

Key words: Agarose gel electrophoresis, Delbergia sissoo L., Genetics analyisis, RAPD.