African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6691

Full Length Research Paper

Introduction of the Sub1 gene into the Russian rice varieties using the polymerase chain reaction (PCR) methods

Elena V. Dubina
  • Elena V. Dubina
  • All-Russian Rice Research Institute, 350921, 3 Belozerny Village, Krasnodar, Russia.
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Andrey V. Alabushev
  • Andrey V. Alabushev
  • Agricultural Research Center, Donskoy, 347740, 3 Nauchniy Gorodok, Zernograd, Russia.
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Pavel I. Kostylev
  • Pavel I. Kostylev
  • Agricultural Research Center, Donskoy, 347740, 3 Nauchniy Gorodok, Zernograd, Russia.
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Yuliya A. Makukha
  • Yuliya A. Makukha
  • All-Russian Rice Research Institute, 350921, 3 Belozerny Village, Krasnodar, Russia.
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Margarita G. Ruban
  • Margarita G. Ruban
  • All-Russian Rice Research Institute, 350921, 3 Belozerny Village, Krasnodar, Russia.
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Ivan V. Balyasnyi
  • Ivan V. Balyasnyi
  • All-Russian Rice Research Institute, 350921, 3 Belozerny Village, Krasnodar, Russia.
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Le H. Ham
  • Le H. Ham
  • Agriculture Genetics Institute (AGI), KM2, Pham Van Dong Road, Bac Tu Liem District, Hanoi, Vietnam.
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Dinh X. Tu
  • Dinh X. Tu
  • Agriculture Genetics Institute (AGI), KM2, Pham Van Dong Road, Bac Tu Liem District, Hanoi, Vietnam.
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Le H. Linh
  • Le H. Linh
  • Agriculture Genetics Institute (AGI), KM2, Pham Van Dong Road, Bac Tu Liem District, Hanoi, Vietnam.
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  •  Received: 20 September 2018
  •  Accepted: 29 October 2018
  •  Published: 29 November 2018

Abstract

Selection done using molecular markers has spread to all types of agricultural plants and animals. Marker assisted selection (MAS) is safe for ecology and food products consumed. The purpose of the study is to develop Sub1A gene (which determines tolerance to prolonged flooding) in the Russian rice varieties using DNA-markers and Polymerase chain reaction (PCR) analysis. A large volume of pre-breeding rice material (F2-F4, BC2F2) was created with tolerance genes for prolonged flooding, to control weed in rice agrophytocenosis. The forms with 120-130-day vegetation period were selected. An analysis of the co-inheritance of simple sequence repeat (SSR) markers Sub1Aq, Sub1C173, Sub1A203 with the Sub1 gene was carried out. It revealed the codominance of the selected microsatellites. They can be used to identify and visualize the allelic state of a given target gene in the breeding material. The recurrent parental rice forms, donors of the gene Sub 1 and the hybrids of F2 and ВС2F1 population were tested on tolerance to prolonged flooding. The gene is effective in ensuring resistance to this stress factor within 2 to 3 weeks and it can be recommended for the breeding programs to develop modern genetic resources of rice tolerant to prolonged and deep flooding for weed control on rice fields.

Key words: Polymerase chain reaction (PCR), rice, Sub genes, tolerance to flooding, weeds.