Abstract

Plant protection is an important area which needs attention since most of the hazardous inputs added into the agricultural system are in the form of plant protection chemicals. Production of the crop is, however, constrained by fungal plant pathogens infections. The present study was also carried out for estimation of total phenols and flavonoids present in fresh faba bean leaf extract with ethanol. Applying the Pseudomonas fluorescens 9 and 10 by bio-primed seed of faba bean treatment enhanced the accumulation of total phenols and flavonoids compared to untreated infected and uninfected untreated faba bean. Bio-priming faba bean seeds with P f9 and P f10 were increased the faba bean total flavonoids in NC 58, Moti and ILB 938 varieties with range 450.58 to 770.19 mg/g compared negative and positive control. The maximum amount of total phenol was recorded 152.25 mg/g in Moti variety with P f9 isolate and 123.70 mg/g was recorded in ILB 938 variety with P f10 isolates. On another hand, all isolates of P. fluorescens were tested for certain biochemical production. Consequently all isolate are positive for all tests. Therefore, from this study it is possible to conclude that the use of P. fluorescens 9 and 10 isolates could reduce the disease incidence and severity of caused by chocolate spot (Botrytis fabae) and simultaneously increased the plant growth promoting and yield performance of faba bean. These isolates can be used as potential biofertilizers, inducer of ant-fungal compounds and also as biocontrol agent against Botrytis fabae. So all isolates of P. fluorescens can be used as plant growth promoting producing bacteria for increasing the yield production and quality of faba bean.  

Key words: Botrytis fabae, faba bean, flavonoids, phenols, Pseudomonas fluorescens.