African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6849

Full Length Research Paper

Use of house cricket to address food security in Kenya: “Nutrient and chitin composition of farmed crickets as influenced by age”

Carolyne Kipkoech
  • Carolyne Kipkoech
  • Department of Food Science and Technology, Jomo Kenyatta University of Agriculture and Technology, P. O. Box 62000-00200, Nairobi, Kenya.
  • Google Scholar
John N. Kinyuru
  • John N. Kinyuru
  • Department of Food Science and Technology, Jomo Kenyatta University of Agriculture and Technology, P. O. Box 62000-00200, Nairobi, Kenya.
  • Google Scholar
Samuel Imathiu
  • Samuel Imathiu
  • Department of Food Science and Technology, Jomo Kenyatta University of Agriculture and Technology, P. O. Box 62000-00200, Nairobi, Kenya.
  • Google Scholar
Nanna Roos
  • Nanna Roos
  • Department of Nutrition, Exercise and Sports,University of Copenhagen, Rolighedsvej 26, 1958, Frederiksberg C, Denmark.
  • Google Scholar

  •  Received: 23 August 2017
  •  Accepted: 21 September 2017
  •  Published: 02 November 2017


House cricket is currently introduced for scaled-up production in farming systems in Kenya and other parts of the world, as an alternative source of animal proteins. The aim of this study was to assess the nutritional composition in farmed cricket as influenced by age in order to ascertain the optimal harvesting time for possible utilization of crickets in improving child nutrition in Kenya. Sampling was carried out between weeks 4 and 13. The moisture content was analysed by drying method, chitin by sodium hydroxide digestion, protein content by estimation of total nitrogen, crude fat by soxhlet extraction method, ash by muffle furnace incineration, available carbohydrates by subtraction, and energy by calculation method. The crude protein mean ranged from 36.00 to 60.00 g/100 g, chitin 2.20 to 12.40 g/100 g, total lipids 12.00 to 25.00 g/100 g, over the 13 weeks period. Minerals concentration was optimum at week 9, with magnesium 1.30 to 11.30 mg/100 g, calcium 1.40 to 19.70 mg/100 g, and zinc 0.20 to 16.60 mg/100 g. Findings from this study indicate that farmed cricket would be best harvested between weeks 9 and 11, when the protein and mineral content is optimum. Nutrients available in farmed crickets show that farmed crickets can be used in child food ingredients to improve child nutrition.

Key words: Farmed crickets, proximate, protein, fatty acid, omega 3, omega 6, minerals, child nutrition.


Food security in Kenya is still a challenge and there is need for agriculture diversity to help Kenyan population get sufficient food (Mburu et al., 2016; Kimiti et al., 2016). Food and nutrition security affects most countries in the world and is directly linked to other problems such as human health and poverty (Pritchard, 2016).
Millions of children in the world are currently affected by acute malnutrition (Bliss et al., 2016; Bartz et al., 2014; Black et al., 2013), with half of the deaths in children below 5 years being caused by under nutrition or nutrition related complications (Black et al., 2013). High prevalence of malnutrition, characterised by stunting early in life has been demonstrated in the Kenyan population by studies done recently, and lack of proteins is rated high in Kenyan children (Kimani-Murage et al., 2015; M’Kaibi et al., 2015). There has also been increased cases in morbidity and mortality due to poverty and food insecurity (Cohen et al., 2015).
While the major source of protein for human consumption is plant and animal proteins (meat, pork, fish, milk and eggs) which is rated higher in quality, and therefore preferred by many people (Ertl et al., 2016; Martens et al., 2013; Cook and Monsen, 1976). These animal proteins are however expensive and not easily available (Van Huis, 2013). The high cost of animal protein is partly contributed by increasing demand for animal protein directly attributed to sharp population rise (Konyole, 2014). This has prompted search for alternative sources which include insects.
Insect consumption has been practiced among Kenyan communities since ancestral times, and more so, when there was food insufficiency (Evans et al., 2015). In the recent years, there is great interest in insect consumption all over the world, since they have been found to contain high nutrient content (van Huis, 2015; Halloran et al., 2015). The only difference in the past and present in relation to edible insects, is that in the past, insects as food was considered a hobby where people in few communities would go to the forest to collect them when the season allowed for non-commercial home consumption compared to the recent times where they are increasingly sold in the markets and on the streets (Durst and Hanboonsong, 2015).
In Kenya, use of insect for food is an ancient tradition in many communities, and currently a more sort after delicacy in the western region of Kenya, (Ayieko et al., 2012; Kinyuru et al., 2009; Christensen et al., 2006). People are used to collecting insects in the wild, this was largely carried out by women and children, and insect farming is thus a new idea among the Kenyan population (Rumpold and Schlüter, 2013; Kinyuru et al., 2012; Bukkens, 1997).
Insects have a varied biochemical composition of both macronutrients and micronutrients (Finke, 2016). Crickets in particular, offer a highly economical protein source (Caparros Megido et al., 2016), and therefore, sustainable solution to existing and looming issues of malnutrition. Better nutrition can be achieved by production and distribution of high quality protein (Kelemu et al., 2015). To help meet growing demands of quality nutrition as the world population grows, insects are good for children  especially  undernourished  children,  due  to high protein and fatty acid content (Acosta and Fanzo, 2012), and plenty of minerals such as iron, selenium, copper, magnesium and zinc. Cricket farming is a feasible venture and has demonstrated success in other countries for example in Thailand (Hanboonsong et al., 2013).
Cricket rearing requires simple locally available raw materials, which includes egg trays, which is importance if the local community will adopt rearing of crickets in an easy affordable way. Currently, cricket farming is still under small-scale production in most  parts of the world (Halloran et al., 2016), but people are slowly adopting to cricket rearing largely for cash at the local markets and this is why there is increased interest in cricket rearing.
Cricket rearing has been shown to be economical in water and feed consumption, since they consume little water and food. Cricket rearing is also time efficient and an environmentally safe way of alternative protein generation as they produce less greenhouse gases (Caparros Megido et al., 2016). Crickets have a high feed conversion rate, therefore providing high quality nutrients, which in turn are cheaper, efficient and environmentally sound protein source when compared with traditional livestock protein sources, which are expensive to maintain (Payne et al., 2015). Crickets could therefore be used to curb food insecurity and malnutrition in developing countries. For this to happen, they should be incorporated in the day to day diet. However, to improve widespread utilization, they have to be reared in large-scale and in a sustainable manner in order to supply a wider population (Van Huis et al., 2015).
The aim of this study was to assess the nutritional and chitin composition of farmed cricket as influenced by age in order to ascertain the optimal harvesting time for maximum nutrient content in an effort to incorporate them in the diets to improve child nutrition. Crickets’ rearing was preferred for this study since crickets are easier and cheaper for mass produce.


Cricket production
This study was carried out at Jomo Kenyatta University of Agriculture and Technology (JKUAT). The rearing was carried out in simple fabricated cages that mimicked the crickets natural environment (Hanboonsong, 2013; Loranger and Bertram, 2016). The nymphs were fed on 21 g/100 g protein feed, commercially available as a starter diet. After two weeks, the diet was alternated with commonly available vegetables and weeds such as pumpkins leaves, cassava leaves, morning glory leaves, black jack and sukuma wiki depending on the availability of the green leaves since crickets are known to be herbivores (Lundy and Parrella, 2015). At four weeks, the 21 g/100 g protein commercial feed was replaced with 14 g/100 g protein commercial feed and supplemented with green leaves for their life span.
For this study, one pen was used to breed crickets under the same structure to minimize  variations  in  environmental  conditions and feeding. All crickets were fed on the same diet and maintained under similar conditions with average temperature of 25°C throughout the study period.
Sample collection and preparation
Sampling was done every week from week four to week 13. Three independent samples were collected weekly on days 1, 3 and 5 of the week. One kilogram of harvested cricket yielded three samples which formed samples representing the week.
After collection, the crickets were frozen at -20°C overnight, then washed in tap water and rinsed in distilled water. The crickets were then oven dried at 50°C for 72 h.  When the crickets were dried with moisture content 4.00 to 6.00g/100g, the crickets were crushed to very small fine particles (250 mm), to get the cricket powder. When 1 kg of the harvested crickets was oven dried, approximately 400 g of dry weight was obtained. The cricket powder was stored at -20°C and during analysis only the required amount was taken from the freezer. All analysis was carried out in triplicate. 
Proximate analysis
Standard methods for proximate analysis were adopted in this study. To determine crude proteins, total nitrogen obtained by micro-Kjeldahl method was used; percentage nitrogen was multiplied by 6.25 N factors to obtain crude protein content in crickets (AOAC, method 990.03). Crude fat was obtained by Soxhlet extraction method (AOAC, method 920.39). AOAC, method 940.28 was used to determine fatty acid profile, and calculation of omega 3 and 6 fatty acid in the crude fat.
Ash determination was done by 550°C muffle furnace incineration (AOAC, method 920.48). To determine the available minerals in the sample, AOAC method 984.27 was used. Mineral analysis was determined by dry ashing and measured by flame atomic absorption spectrophotometry (Shimadzu 6300 AAS AA/AE Spectrophotometer) (AOAC, 2005). Quantification of minerals was done using commercial standards (Sigma-Aldrich Chemie, Steinheim, Germany). In-house controls were also used by using a vacuum packed controlled sample stored at -20°C.
Available carbohydrate content was calculated from the proximate values by subtraction method. Energy levels from the proximate analysis, was obtained by Energy content = (Crude protein × 4) + Crude fat × 9) + ( Available carbohydrate × 4) (AOAC, 2005).
Chitin extraction
To extract chitin from crickets, 150 g of ground cricket powder was placed in 1000 ml beaker containing 4% boiling sodium hydroxide and the mixture was boiled for 2 h for the powder to undergo deproteinization to remove sugar and protein components (Toan, 2009; Lertsutthiwong et al., 2002). The content was allowed to cool for 30 min, followed by centrifuging at 10000 revolutions per minute, for 10 min to separate the solid part containing chitin from the liquid part containing sodium hydroxide, sugar and proteins components (Galed et al., 2005).
To dissolve minerals from the centrifuged residue, 600 ml of 1% hydrochloric acid was added and the residue placed in a rotor shaker (Sanyo electric company limited, Japan) rotating at 40 rpm for 24 h at room temperature (Trung et al., 2006). The content was then subjected to centrifugation and the liquid part disposed while the remaining residue was treated with 500 ml of 2% boiling sodium hydroxide for 1 h to decompose albumin (Puvvada et al., 2012).
The content underwent centrifugation and the liquid part that contained albumin was discarded. The brown solid residue was washed three times with boiled distilled water to remove the remaining polysaccharides and sodium hydroxide, and then dried at 70°C for 60 h after which the extracted chitin was kept in zip lock bags at -20°C until use.
Amino acid analysis
Approximately, 2.00 g of the cricket flour sample was used for amino acid analysis. The sample was defatted using methanol before hydrolysing using 6 M hydrochloric acid. Amino acid analysis was done using ion exchange chromatography, the hydrolysed sample was injected into the amino acid analyser (Technicon Instrumentation Corporation, Dublin, Ireland.), for separation and characterization of amino acids, using the Technicon sequential multi sample amino acid analyser (Ertingshausen et al., 1969).


Figure 1, shows the nutrient and energy content of cricket based on harvesting age. Cricket which were four weeks old were chosen as the starting point for cricket harvesting since crickets which were less than four weeks old were too small to be handled, and this would mean harvesting so many crickets to obtain the required mass. Protein content was on a staggering rise from week 4 to 11 and dropped at weeks 12 and 13. The protein content in cricket increased as the cricket aged, though the increase is not linear and maximum protein content was seen between week 9 (58.30 g/100 g), week 10 (59.74 g/100 g) and week 11 (60.40 g/100 g). The rise in total lipids is slow when compared with cricket protein content rise (Figure 1), the lowest lipid content was seen at week 13 (12.00 g/100 g), while the highest was at weeks 9 and 10 at 25 g/100 g. Cricket chitin content was on steady rise as the crickets aged and at week 13 chitin content was at maximum 12.33 g/100 g, of the total cricket content. Ash content was almost constant across all ages with approximate composition of between 4.00 g/100 g and 5.00 g/100 g content; there was no defined rise in ash content. Initially, available carbohydrate was high at 39.23 g/100 g at four weeks, then drops drastically to 14.60 g/100 g at week 6, before increasing again at week 7 (Figure 1). Energy levels were high between 1827.03 kJ/100 g at week 4 and rose to 1807.49 kJ/100 g at week six. Optimum energy content is seen at week nine, 1774.02 kJ/100 g. 
Table 1 shows mineral content in farmed crickets; mineral content starts at a higher level and slightly drops as the crickets aged and finally rose at weeks 11, 12 and 13. There is no defined rise or drop in mineral contents: magnesium optimum levels of 12.00 mg/100 g was seen at week 12, calcium 11.33 mg/100 g at week 13, copper 10.67 mg/100 g at week 12, iron at 0.04 mg/100 g at week 13, and zinc 19.33 mg/100 g at week 11.
Figure 2 outlines the major fatty acid fractions of cricket oil, which clearly show that cricket fat is rich in good fats which includes omega 6 and 3. Crickets had  polyunsaturated fatty acids (PUFAs) ranging from 2.00 to 5.00 g/100 g, the PUFAs increased from week 4 to reach a maximum of 5.00 g/100 g at week 7 and drops again to 3.00 g/100 g at week 13 (Figure 2). Saturated fatty acid (SATU’s) increased as the cricket aged from 48.29 g/100 g at week 4 to 63.88 g/100 g at week 6 and then drops to 46.12 g/100 g at week 13. Mono-unsaturated fatty acid (MUFAs) increased from week 4 to 12 and slightly reduces at week 13.
Table 2 shows the fatty acid composition of cricket oil at different ages. When cricket fatty acid profile is further grouped into omega 3 and 6 and the ratio between omega 6 and 3 calculated, the ratio ranged from 0.33 to 2.40. 
Table 3 shows the amino acid content of cricket protein in mg/g protein. Out of the total amino acids, 13  amino acids were analysed and 7 out of the 13 had detectable values.


This study has demonstrated that house cricket has high protein content up to about 60.67 g/100 g of the total nutrient content, based on daily value in 8368.00 kJ diet for children above four years and adults (Krauss et al., 2000; Mann and Truswell, 2012); 100 g of cricket serving per day would provide the required 50 g protein. Since the crickets were fed on locally available diets, it is clear that the high protein content can be achieved by local farmers who would like to adopt the simple diet and this makes cricket rearing for protein supplementation a venture worth exploitation in Kenya and other part with similar setups as Kenya. Most children suffer from malnutrition due to insufficient or lack of proteins and therefore crickets provide an opportunity to help these children.
The results correlated well with other studies that have shown high protein content in insects (Kinyuru et al., 2015; Collavo et al., 2005). Since protein content declined in weeks 12 and 13, it would be of importance to consider weeks 9, 10 and 11 have the optimal time for cricket harvesting if the target is to get the highest protein content. In a study carried out by De Foliart et al. (1982), in the earlier decades, crickets were found to contain high protein content of 54.00 g/100 g, this study has demonstrated that cricket protein content can be higher than 54 g/100 g.
Crude fat content of between 12.67 and 25.00 g/100 g in house cricket, is within the range observed by Narzani and Sarmah (2015) where crude fat content in insect seemed to vary from 1.00 g/100 g to 40.00 g/100 g (Narzari and Sarmah, 2015). If children would be given 100 g serving of crickets per day, the crude fat content would provide about 38% of the total daily requirements (65 g/100 g). Other studies have shown low fat content in crickets, when compared with fat content in termites (Payne et al., 2015). Low fat content in cricket would be of importance for industrial exploitation of cricket, since high fat content hinders procesability and shelf life of food products, hence reduce its mass production and market potential (Kinyuru, 2014). Crickets therefore demonstrate high market potential and extended shelf life when compared with termites in relation to the overall fat content and their inclusion in food to supplement protein and fat especially in complementary food.
Insects are known to contain chitin as the major fibre, and therefore, in the current study, chitin was quantified from crickets. Chitin content in insects has been found to range from 4.00 g/100 g to 21.00 g/100 g (Finke, 2015). In the current study, the chitin content ranged between 2.00 g/100 g and 12.00 g/100 g which is the lowest at the tender age and increases as cricket age. This would be the highest contributor of hardened wings as the crickets’ age increase. Cricket harvested at weeks 12 and 13 with the highest chitin content were hard to process since the cuticle was rigid and therefore the powder obtained was rough 510 mm as compared to 250 mm in weeks 4 to 11. Rough cricket flour would greatly affect the end products made from cricket powder and therefore it would be better to harvest crickets before too much hardening of the cuticle between weeks 9, 10, and 11. Chitin also did not contribute to energy content and the content rises, while the energy content declined, and this is why at weeks 12 and 13 the energy content was low.
Ash content was below 10.00 g/100 g in the current study, recent studies on insects have indicated that the ash content in insect is less than 10.00 g/100 g  (Rumpold and Schlüter, 2015). In the past, studies have demonstrated that crickets having varied in nutrient content (Belluco et al., 2013), with content of available carbohydrates ranging from 1.00 g/100 g to 47.00 g/100 g (Narzari and Sarmah, 2015). In the current study, carbohydrate content was within this range, and as the age increased, the carbohydrate levels steadily decrease over time especially when protein levels and chitin content increased. At week 7, there is a drop in most nutrients which seem to be the lowest at this age; it would be of importance for future scientist to find out if the drop has any correlation with start of egg laying in cricket.
Previous studies have shown insects to be energy dense with up to 2418.35 kJ/100 g of energy (Finke, 2013). The energy content fluctuates, which is attributed majorly to the fluctuations in other proximate contents. The energy levels in cricket started off at a high level and when fat content drops the energy content also drop. As much as the energy contents drops, the drop is not much since the overall energy content ranges from 370.01 kJ/100 g and 1807.49 kJ/100 g. This study shows crickets are energy dense food source, and can easily be an option to curb malnutrition in energy deficient children in developing world where malnutrition rates are still high (Black et al., 2013), as long as the crickets are harvested at the right age to ensure a balance of proximate nutrients, minerals and energy.
Cricket mineral composition shows that crickets are not just energy dense food, they are also good for curbing high mineral deficiency in Kenyan children, since past studies have indicated high mineral deficiency in children (Duong et al., 2015). The data obtained from this study compared well with other studies that have analysed mineral content of crickets (Rumpold and Schlüter, 2015; Payne et al., 2016; Taufek et al., 2016).
Crickets oil contains lauric acid 1.5% which is found in breast milk and coconut oil and is converted to monolaurins which protects children from disease (Dayrit, 2015). Crickets contain myristic acid 3% which is also contained  in  cow  milk  (Mazhitova  et  al.,   2015).   Poly unsaturated fatty acid (PUFAs) content in crickets is an advantage especially in human nutrition since PUFAs in diet has been linked to anti-obesity and increased activity in children (Åšrednicka-Tober et al., 2016). Cricket meal would provide higher percentage of saturated fatty acid at 46 g/100 g when compared with 33 g/100 g in beef and 34 g/100 g in chicken (Ton et al., 2015), while PUFAs was at 5 g/100 g in crickets which is the same as PUFAs percentage in beef (Ton et al., 2015; Åšrednicka-Tober et al., 2016). Supplementing children diet with cricket would give the children the needed lipids which would ensure more health and increased activity in children.
Oil containing omega 3 and 6 fats has been rated positive in child’s diet. The oil found in cricket has omega 3 and 6 fatty acid which clearly indicates that feeding children with cricket would boost their good fat content. Past studies have indicated that fatty acid is more desirable when the ration of omega 6:3 is lower or almost equal to one (Yang et al., 2006);  human beings are believed to  have evolved on omega 6:3 balanced diet of 1:1 (Oddy et al., 2004) and increase ratio of omega 6:3 promotes obesity in children (Simopoulos, 2016). Growing children need sufficient amounts of omega 3 and 6 (Brenna et al., 2015). Therefore, cricket meal is important if adopted to feed children to improve child nutrition.
There is need for higher protein content in children to be able to meet the required amino acid patterns. Malnourished children require slightly higher protein intake, to enable them get required amino acids (Ricardo et al., 2015). Since cricket contain high protein content, its use in improving child nutrition is likely to boost the amino acid requirements in moderately malnourished children. Farming of crickets can be achieved in drought stricken areas, because cricket requires small amount of space and water. Since drought is a major constrain in Kenyan agriculture (Huho and Mugalavai, 2010), exploitation of crickets can address food security.


The optimum age for farmed cricket harvesting would be between week 9, 10, and 11, when the protein, total lipids and mineral content is optimum. At this age chitin content is slightly lower hence better procesability and finer flour which would greatly enhance the end product made from cricket. Cricket omega 6 to 3 ratio makes cricket oil good for child nutrition. This study recommends the rearing of crickets and the use of cricket in child nutritional interventions for its high protein content, which is often the major cause of child under nutrition.


The authors have not declared any conflict of interests.


Acosta AM, Jessica F (2012). Fighting maternal and child malnutrition: analysing the political and institutional determinants of delivering a national multisectoral response in six countries. A synthesis paper. Brighton, UK, Institute of Development Studies.


AOAC (2005). Official method of analysis. (18th ed.)Association of Officiating Analytical Chemists, Washington, DC method 997.08.


Ayieko MA, Kinyuru JN, Ndong'a MF, Kenji GM (2012). Nutritional value and consumption of black ants (Carebara vidua Smith) from the Lake Victoria region in Kenya. Adv. J. Food Sci. Technol.


Bartz S, Aaloke M, Christoph H, James B, Michael M, Tonny K, Elizabeth K, Robert S, John Bt, John V, Peter ST (2014). Severe acute malnutrition in childhood: hormonal and metabolic status at presentation, response to treatment, and predictors of mortality. J. Clin. Endocrinol. Metab. 99(6):2128-2137.


Belluco S, Carmen L, Michela M, Cristiana CA, Maurizio GP, Antonia R (2013). Edible insects in a food safety and nutritional perspective: a critical review. Comprehensive Rev. Food Sci. Food Saf. 12(3):296-313.


Black RE, Cesar GV, Susan PW, Zulfiqar AB, Parul C, Mercedes De O, Majid E, Sally G-M, Joanne K, Reynaldo M (2013). Maternal and child undernutrition and overweight in low-income and middle-income countries. Lancet 382(9890):427-451.


Bliss JR, Martin N, Rebecca JS, David LP (2016). Stigma as a barrier to treatment for child acute malnutrition in Marsabit County, Kenya. Matern. Child Nutr. 12(1):125-138.


Brenna JT, Peter A, Paluku B, James AB, Philip CC, Kelsey DJ, Lei L, Mark M, Indi T, André B (2015). Balancing omega-6 and omega-3 fatty acids in ready-to-use therapeutic foods (RUTF). BMC Med. 13(1):1.


Bukkens SGF (1997). The nutritional value of edible insects. Ecol. Food Nutr. 36(2-4):287-319.


Caparros M, Rudy TA, Clément N, Christophe B, Sabine D, Jan B, Éric H, Frédéric F (2016). Optimisation of a cheap and residential small‐scale production of edible crickets with local by‐products as an alternative protein‐rich human food source in Ratanakiri Province, Cambodia. J. Sci. Food Agric. 96(2):627-632.


Christensen DL, Francis OO, Michael NM, Torben L, Henrik F, Jens A-H (2006). Entomophagy among the Luo of Kenya: a potential mineral source? Int. J. Food Sci. Nutr. 57(3-4):198-203.


Cohen CR, Rachel LS, Elly W, Elizabeth AB, Abigail MH, Stephen S, Richard R, Kate MS, Lisa MB, Phelgona O (2015). Shamba Maisha: pilot agricultural intervention for food security and HIV health outcomes in Kenya: design, methods, baseline results and process evaluation of a cluster-randomized controlled trial. SpringerPlus 4(1):122.


Collavo A, Glew RH, Huang YS, Chuang LT, Bosse R, Paoletti MG (2005). House cricket small-scale farming. Ecological implications of minilivestock: potential of insects, rodents, frogs and snails. pp. 519-544.


Cook JD, Elaine RM (1976). Food iron absorption in human subjects. III. Comparison of the effect of animal proteins on nonheme iron absorption. Am. J. Clin. Nutr. 29(8):859-867.


Dayrit FM (2015). The properties of Lauric acid and their significance in coconut oil. J. Am. Oil Chem. Soc. 92(1):1-15.


De Foliart GR, Finke MD, Sunde ML (1982). Potential value of the Mormon cricket (Orthoptera: Tettigoniidae) harvested as a high-protein feed for poultry. J. Econ. Entomol. 75(5):848-852.


Duong Minh-Cam, Mercedes Mora-Plazas, Constanza M, Eduardo V (2015). Vitamin B-12 Deficiency in Children Is Associated with Grade Repetition and School Absenteeism, Independent of Folate, Iron, Zinc, or Vitamin A Status Biomarkers. J. Nutr. 145(7):1541-1548.


Durst PB, Hanboonsong Y (2015). Small-scale production of edible insects for enhanced food security and rural livelihoods: experience from Thailand and Lao People's Democratic Republic. J. Insects Food Feed 1(1):25-31.


Ertingshausen Gerhard, Harvey JA, Allen SR (1969). Fully automated high-speed ion-exchange chromatography of amino acids. J. Chromatogr. A 42:355-366.


Ertl P, Knaus W, Zollitsch W (2016). An approach to including protein quality when assessing the net contribution of livestock to human food supply. Animal 10(11):1883-1889


Evans J, Mohammed HA, Roberto F, Michael BF, Halloran A, Annette BJ, Gabriela M-V, Meyer-Rochow VB, Münke-Svendsen C, Søren BO (2015). Entomophagy': an evolving terminology in need of review. J. Insects Food Feed 1(4):293-305.


Finke MD (2013). Complete nutrient content of four species of feeder insects. Zoo Biol. 32(1):27-36.


Finke MD (2015). Complete nutrient content of four species of commercially available feeder insects fed enhanced diets during growth. Zoo Biol. 34(6):554-564.


Finke MD (2016). Diet for altering the nutrient composition of feeder Insects. US Patent 20,160,058,055.


Galed G, Beatriz M, Inés P, Alejandro S, Ángeles H (2005). N-Deacetylation and depolymerization reactions of chitin/chitosan: Influence of the source of chitin. Carbohydr. Polym. 62(4):316-320.


Halloran A, Roos N, Flore R, Hanboonsong Y (2016). The development of the edible cricket industry in Thailand. J. Insects Food Feed pp.1-10.


Halloran A, Paul V, Hanboonsong Y, Ekesi S (2015). Regulating edible insects: the challenge of addressing food security, nature conservation, and the erosion of traditional food culture. Food Secur. 7(3):739-746.


Hanboonsong Y, Tasanee J, Patrick BD (2013). Six-legged livestock: edible insect farming, collection and marketing in Thailand. 



Huho JM, Edward MM (2010). The effects of droughts on food security in Kenya. Int. J. Clim. Change: Impacts and Responses 2(2):61-72.


Kelemu S, Niassy S, Torto B, Fiaboe K, Affognon H, Tonnang H, Maniania NK, Ekesi S (2015). African edible insects for food and feed: inventory, diversity, commonalities and contribution to food security. J. Insects Food Feed 1(2):103-119.


Kimani-Murage EW, Stella KM, Samuel OO, Martin KM, Steven Van de V, Catherine K (2015). Evidence of a double burden of malnutrition in urban poor settings in Nairobi, Kenya. PloS one 10 (6):e0129943.


Kimiti JM, Simon WM, Gilbert K, Omwoyo O, John MM, Ezekiel MN (2016). Agrobiodiversity conservation enhances food security in subsistence-based farming systems of Eastern Kenya. Agric.Food Secur. 20165:19. 



Kinyuru JN., Kenji GM, Njoroge MS (2009). Process development, nutrition and sensory qualities of wheat buns enriched with edible termites (Macrotermes subhylanus) from Lake Victoria region, Kenya. Afr. J. Food Agric. Nutr. Dev. 9:1739-1750.


Kinyuru JN, Silvenus OK, Glaston MK, Christine AO, Victor OO, Bethwell OO, Benson BE, Henrik F, Nanna R (2012). Identification of traditional foods with public health potential for complementary feeding in western Kenya. J. Food Res. 1(2):148-158.


Kinyuru JN, Joseph BM, Chris AR, Nancy WN (2015). Edible insects—a novel source of essential nutrients for human diet: Learning from traditional knowledge. Anim. Front. 5(2):14-19.


Kinyuru JN (2014). Development and evaluation of complementary foods based on traditional foodstuffs in Western Kenya. 


Konyole SO (2014). Effect of improved complementary foods on growth and iron status of Kenyan infants. University of Nairobi.


Krauss RM, Robert HEckel, Barbara H, Lawrence JA, Stephen RD, Richard JD, John WE, Penny K-E, Ira JG, Theodore AK (2000). AHA Dietary Guidelines. Circulation 102(18):2284-2299.


Lertsutthiwong P, Ng Chuen H, Suwalee C, Willem FS (2002). Effect of Chemical Treatment on the Characteristics of Shrimp Chitosan. J. Metals Mat. Miner. 12(1):11-18.


Loranger MJ, Susan MB (2016). The effect of male dominance on female choice in a field cricket (Gryllus assimilis). Anim. Behav. 114:45-52.


Lundy ME, Michael PP (2015). Crickets are not a free lunch: protein capture from scalable organic side-streams via high-density populations of Acheta domesticus. PloS one 10(4):e0118785.


M'Kaibi FK, Nelia PS, Sophie O, Lisanne Du P (2015). Effects of agricultural biodiversity and seasonal rain on dietary adequacy and household food security in rural areas of Kenya. BMC Public Health 15(1):422.


Mann J, Stewart T (2012). Essentials of human nutrition: Oxford University Press. 716p.


Martens SD, Tassilo TT, Jérôme B, Michael P, Carlos EL (2013). Alternative plant protein sources for pigs and chickens in the tropics–nutritional value and constraints: a review. J. Agric. Rural Dev. Trop. Subtropics (JARTS) 113(2):101-123.


Mazhitova AT, Kulmyrzaev AA, Ozbekova ZE, Bodoshev A (2015). Amino Acid and Fatty Acid Profile of the Mare's Milk Produced on Suusamyr Pastures of the Kyrgyz Republic During Lactation Period. Procedia-Social Behav. Sci. 195:2683-2688.


Mburu SW, Gilbert K, Jacinta MK, Omwoyo O, John MM, Ezekiel MN (2016). Agrobiodiversity conservation enhances food security in subsistence-based farming systems of Eastern Kenya. Agric. Food Secur. 5(1):19.


Narzari S, Jatin S (2015). Proximate composition of wild edible insects consumed by the Bodo tribe of Assam, India. Int. J. Bioassays 4(07):4050-4054.


Oddy WH, NH De Klerk, Kendall GE, Mihrshahi S, Peat JK (2004). Ratio of Omega‐6 to Omega‐3 Fatty Acids and Childhood Asthma. J. Asthma 41(3):319-326.


Payne CLR, Peter S, Mike R, Kenichi N (2016). A systematic review of nutrient composition data available for twelve commercially available edible insects, and comparison with reference values. Trends Food Sci. Technol. 47:69-77.


Payne CLR, Scarborough P, Rayner M, Nonaka K (2015). Are edible insects more or less 'healthy' than commonly consumed meats & quest; A comparison using two nutrient profiling models developed to combat over-and under nutrition. Eur. J. Clin. Nutr. 70:285-291


Pritchard B (2016). Food and nutrition security. Routledge Handbook of Food and Nutrition Security:1.


Puvvada YS, Saikishore V, Sudheshnababu S (2012). Extraction of chitin from chitosan from exoskeleton of shrimp for application in the pharmaceutical industry. International Current Pharm. J. 1(9):258-263.


Ricardo U, Anura K, Tano-Debrah K, Grant AA, Yasuhiko T, Shibani G (2015). Role of protein and amino acids in infant and young child nutrition: Protein and amino acid needs and relationship with child growth. J. Nutr. Sci. Vitaminol. 61(Supplement):S192-S194.


Rumpold BA, Oliver S (2015). Insect-based protein sources and their potential for human consumption: Nutritional composition and processing. Anim. Front 5(2):20-24.


Rumpold BA, Oliver KS (2013). Nutritional composition and safety aspects of edible insects. Mol. Nutr. Food Res. 57(5):802-823.


Simopoulos AP (2016). An increase in the omega-6/omega-3 fatty acid ratio increases the risk for obesity. Nutrients 8(3):128.


Åšrednicka-Tober D, Marcin B, Chris JS, Roy S, Charles B, Håvard S, Joanna G-O, Ewa R, Krystyna S-S, Mick E (2016). Higher PUFA and n-3 PUFA, conjugated linoleic acid, α-tocopherol and iron, but lower iodine and selenium concentrations in organic milk: a systematic literature review and meta-and redundancy analyses. Br. J. Nutr. 115(06):1043-1060.


Taufek NM, Firdaus A, Hasniyati M, Ameenat AR, Shaharudin AR, Zazali A (2016). The effect of dietary cricket meal (Gryllus bimaculatus) on growth performance, antioxidant enzyme activities, and haematological response of African catfish (Clarias gariepinus). Fish Physiol. Biochem. 42(4):1143-1155.


Toan NV (2009). Production of chitin and chitosan from partially autolyzed shrimp shell materials. Open Biomat. J. 1:21-24


Ton S, Massimo De M, Davide M, Michele M, Martino C, Mauro P (2015). Use of near infrared technology to predict fatty acid groups in commercial ground meat products. Poljoprivreda 21(1 Supplement):232-236.


Trung T Si, Wah WT-H, Nguyen TQ, Chuen-How Ng, Willem FS (2006). Functional characteristics of shrimp chitosan and its membranes as affected by the degree of deacetylation. Bioresour. Technol. 97(4):659-663.


van Huis A (2013). Potential of insects as food and feed in assuring food security. Ann. Rev. Entomol. 58:563-583.


Van Huis A, Dicke M, Van Loon JJA (2015). Insects to feed the world. J. Insects Food Feed 1(1):3-5.


Van Huis A (2015). Edible insects contributing to food security? Agric. Food Secur. 4(1):1.


Yang Li‐Feng, Sirithon S, Duo Li (2006). Polyunsaturated fatty acid content of edible insects in Thailand. J. Food Lipids 13(3):277-285.