Development of agro-technique is one of the basic conservation strategies for sustainable development and use of plant wealth. Keeping in view the immense importance of agro-techniques the present study was carried out to develop agro-techniques for an important orchid species (Dactylorhiza hatagirea (D.Don) Soo.) so as to conserve this prized plant species under ex- situ conditions.Present study revealed that mixture of sand, soil, coke, rice straw, Brick chips Sphagnum moss, Betula bark was suitable for successful establishment of this plant species. Timing of transplantation and proper irrigation prove crucial for establishment of this plantspecies upon transplantation.It has also been observed thattreatment of tubers with different concentrations of gibberlic acid prior to their transplantation results into increase in shoot elongation, number of leaves and flowers also increases. The present communication will prove helpful for mass cultivation of this plant species under ex-situ conditions.

Keywords: Agro-techniques, successful establishment, transplantation, ex-situ conservation