The present study was aimed at investigating the antioxidant activities of the methanolic extract of pomegranate (Punica granatum) fruit. The antioxidant activities of extract have been evaluated by using in vitro assays and in vivo model. In case of in vitro studies, the antioxidant activity of methanolic extract of pomegranate fruit was 198.31 mg/100g; 192.25 mg/100g; 181.13 mg/100g and 143.24 mg/100g by DPPH, nitric oxide, superoxide radical scavenging assays and ferric reducing antioxidant power assay, respectively. In case of in vivo studies, the levels of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and lipid peroxidation (LPO) were restored towards the normal value in fruit supplemented alloxan induced diabetic rats. The free radical scavenging and antioxidant activities may be attributed to the presence of total poly phenols (148.61 mg/100g), total flavonoids (209.83 mg/100g) and ascorbic acid content (24.21 mg/100g) present in pomegranate fruit. The results obtained in the present study indicate that pomegranate fruit is a potential source of natural antioxidant. Keywords: Pomegranate, Radical scavenging activity, Reducing power, Antioxidant enzymes, Lipid peroxidation

Keywords: Pomegranate, Radical scavenging activity, Reducing power, Antioxidant enzymes, Lipid peroxidation