Nowadays more attention has been given to mushroom production as new sources of nutrients, medicinal uses; especially for degenerative diseases and environmental sustainability through solid organic waste recycling. The yield of oyster mushroom (Pleurotusostreatus) grown on the substrate composed from creeping bent grass (Agrostis sp.), waste paper, wheat bran supplemented with cotton seed waste indicated the positive response. The experimental design constitutes ten treatments with different relative quantities of cottonseed waste and creeping bent grass, in three replicates from the middle of March 2018 to the end of May 2018. Fastest mycelia colonization was observed in the treatments where less than 50 % of cottonseed waste was substituted. Relatively, longest production cycle and lowest yields were observed for treatments with the highest rate of Agrostis sp, but the final biological efficiency (197 %) remained at a high level. The study reveals that mixing of creeping bent grass (Agrostis sp.) biomass together with different proportion of cotton seed waste, and constant parts of wheat bran and waste paper as substratum is feasible for growing the oyster mushroom.

Keywords: Creeping bent grass, cotton seed waste, oystermushroom, waste paper, yield