African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 6 March 2012; 11(19)

March 2012

Progress in structural analysis of glycosaminoglycans and their applications in biomaterials

  The rising interest in the application of glycosaminoglycans (GAGs) is one of the main reasons for exploring different species and optimizing the extracting conditions of various GAGs in the last decade. Recent research data on GAGs have suggested that they have many new biological functions such as anti-atherogenesis, anticoagulation, prevention and cure of arthritis, morphogenesis and cell division. They...

Author(s):   Shuangli Xiong, Anlin Li, and Dabin Hou        

March 2012

Changes in germination characteristics and seedling growth between storage and non-storage of primed tall fescue seed

  In seed priming studies, seeds usually are sown directly in the field or stored for short periods of time. The objective of this study was to determine the effects of storage on the primed seed of tall fescue. Hydropriming and osmopriming methods were used in this study to prime the seeds. The water osmotic potential for osmopriming was between -1.5 and -2.2 MPa. After drying, the seeds were first...

Author(s):   Behzad Behtari and Ghasem Ali Dianati Tilaki        

March 2012

Genetic diversity and identification of variety-specific AFLP markers in fenugreek (Trigonella foenum-graecum)

  Fenugreek (Trigonella foenum-graecum), an important spice crop belongs to the family Fabaceae. India is the largest producers as well as the largest exporter of fenugreek. Five common fenugreek varieties of India were included in this study for molecular analysis. For randomly amplified polymorphic DNA (RAPD) analysis, a total of 47 bands in the size range of 200 to 5000 bp were produced by examining...

Author(s): Vinay Kumar, Navin Srivastava, Abhishek Singh, M. K. Vyas, Shobit Gupta, Kalpesh Katudia, Kanak Vaidya, Spandan Chaudhary, Arpita Ghosh and Surendra K Chikara

March 2012

Genetic polymorphism in β-lactoglobulin gene of some sheep breeds in the Kingdom of Saudi Arabia (KSA) and its influence on milk composition

  β-Lactoglobulin (β-Lg) is one of the most important proteins in mammals’ milk. It plays a crucial role in milk quality. The polymorphism of β-Lg gene can be used as a marker system. To analyze the genotype distribution of β-Lg gene in some sheep breeds reared in Taif region of Saudi Arabia and its influence on milk composition, sixty (60)animals...

Author(s):   Samir A. El-Shazly, Magdy E. Mahfouz, Saad A. Al-Otaibi and Mohamed M. Ahmed,      

March 2012

Tissue culture-induced alteration in cytosine methylation in new rice recombinant inbred lines

  Zizania DNA introgression could induce a large number of genetic and epigenetic changes of the new rice recombinant inbred lines genome. In this present study, we employed inter-simple sequence repeat (ISSR) to further study the genetic and epigenetic changes that are induced by tissue culture. Changes induced by tissue culture are mostly epigenetic. One kind of methylation alterations is that...

Author(s):   Xiaohui Shan, Yidan Li, Mei Tan and Qing Zhao      

March 2012

Polyethylene glycol (PEG)-mediated transformation of the fused egfp-hph gene into Pleurotus ostreatus

  A transformation system for the basidiomycete Pleurotus ostreatus was established using PEG-mediated transformation. The homologous glyceraldehyde-3-phosphate dehydrogenase gene (gpd) promoter and terminator regions of P. ostreatus were amplified and cloned onto the restriction site of the pUC19 vector. Enhanced green fluorescent protein-encoding gene (egfp) and a selection marker, the...

Author(s):   Y. Liu, S.X. Wang, Y.G. Yin, S. Zhao, X.L. Geng and F. Xu        

March 2012

Antibacterial activity of the endophytic fungi from medicinal herb, Macleaya cordata

  A total of 48 endophytic fungal isolates were separated from the healthy roots ofMacleaya cordata R. Br. (Papaveraceae), a traditional medicinal herb mainly distributed in China. Nine distinct isolates (Macof01 to Macof09) were selected for further taxonomical identification by morphological traits and internal transcribed spacer (ITS) rRNA gene sequence analysis. Seven genera...

Author(s):   Tijiang Shan, Weibo Sun, Jingfeng Lou, Shan Gao, Yan Mou and Ligang Zhou        

March 2012

Symbiotic responses to insoluble phosphorus supply in common bean (Phaseolus vulgaris L.): Rhizobia symbiosis

  The common bean (Phaseolus vulgaris L.) is often grown under various environmental constraints in soil as well as phosphorus (P) deficiency. In worldwide soils, P massively exists in insoluble forms especially in acidic and basic soils, where it is greatly combined with Al, Fe and Ca ions hydroxide. In order to study the effect of insoluble phosphorus (IP) in hydroponic culture, two common bean...

Author(s):   Abdi Neila, Bargaz Adnane, Bouraoui Manel, Ltaief Boulbaba, Ghoulam Cherki and Sifi Bouaziz      

March 2012

CRSM-38, a new high yielding coupled with CLCuV tolerance cotton (Gossypium hirsutum L.) variety

  CRSM-38 is a high yielding and leaf curl virus tolerant cotton variety, developed in Pakistan.  Hybridization and subsequent pedigree method’s selection work was carried out under natural field conditions at Cotton Research Station, Multan during the year 2001 and 2002 by using a virus tolerant strain (583-85/99) and a variety (FH-900). The new variety exhibited an average of 77.9% higher degree of...

Author(s):  Saghir Ahmad, Altaf Hussain, Mamoona Hanif, Khalid Mahmood, Wajad Nazeer, Abid Mahmood, Noor-ul-Islam, Waqas Malik, Abdul Qayyum and Kanwal Hanif      

March 2012

Oxidative damage and antioxidant response caused by excess copper in leaves of maize

  Adequate copper (Cu2+) concentrations are required for plants, but at higher concentrations it can cause toxic effects. In this study, physiological parameters and antioxidant enzymes were assayed in the leaves of maize variety Zhendan958, which is one of the dominant cultivar of maize (Zea mays L.) in China due to its better traits in production, after the seedling was treated with 2 to 8 mM Cu for 96...

Author(s):   Li Hong Nie, Yan Zhao Wang ,Wei Pin Fang, De Yi Xie, Shuang Gui Tie, Zhong Jie Tang, Yuan Ming Zhao and Wu Li        

March 2012

Dormancy-breaking and salinity/water stress effects on seed germination of Atlas cypress, an endemic and threatened coniferous species in Morocco

  Atlas cypress (Cupressus atlantica Gaussen) is an endemic coniferous medicinal species geographically restricted to the N’Fis valley river in the High Atlas Mountains in South-Western Morocco. Eight dormancy-breaking treatments, 5 NaCl concentrations, and 5 water potentials were tested on the germination of Altas cypress seeds that had...

Author(s):   Youssef Sfairi, Ouahmane Lahcen, Mohamed Najib Al Feddy and Abdelaziz Abbad      

March 2012

Assessment of washing procedure for determination some of airborne metal concentrations

  This study was proposed to assess the suitability of washing technique to distinguish between airborne and soil borne several metal contaminants. For this reason, six plant species which grew under Mobarakeh Steel Company emissions were selected. Aluminum, iron, nickel, manganese, zinc, copper and lead concentrations were determined by inductively coupled plasma atomic emission spectroscopy (ICP-AES) in...

Author(s):   M. Ataabadi, M. Hoodaji and P. Najafi        

March 2012

Oil palm empty-fruit bunch application effects on the earthworm population and phenol contents under field conditions

  The amounts of polyphenols at several stages of oil palm empty-fruit bunch (EFB) composting, the types of phenol compounds in several types of EFB composting processes and the effects of phenol compounds on EFB composting in an earthworm population were evaluated under field conditions. The amount of extractable phenols from decomposed EFB increased at the early stage of decomposition and decreased with...

Author(s):   D. T. Sabrina, A. W.  Gandahi, , M. M. Hanafi, T. M. M. Mahmud, A. A. Nor Azwady      

March 2012

Diversity of isolates performing Fe(III) reduction from paddy soil fed by different organic carbon sources

  This study examined microbial diversity of Fe(III)-reducing bacteria isolates in paddy soils amended with ferrihydrite and different organic carbon (C) sources. Results show that Fe(III) reduction rate and Fe(II) accumulation in soil enrichments amended with glucose were the greatest followed by pyruvate, lactate, acetate and control. ARDRA (amplified ribosomal deoxyribonucleic acid restriction...

Author(s):   Weijie Yi, Baoli Wang and Dong Qu        

March 2012

Isolation and characterization of Pseudomonas resistant to heavy metals and poly aromatics hydrocarbons (PAHs) from Persian Gulf sediments

  The Persian Gulf is the most strategic waterway in the world due to its importance in the global oil transportation. Heavy metals and poly aromatics hydrocarbons (PAHs) are the most important pollutants in the Persian Gulf and other marine ecosystems. Our goal in this study is to isolate high resistant degrading species for copper and phenanthrene from Khur Mousa sediments (located in the North of the Persian...

Author(s):   Alireza Safahieh, Hajar Abyar, Zohreh Roostan, Hossein Zolgharnein and Fatemeh Mojoodi        

March 2012

Construction of recombinant Pichia strain GS115-Ch-Glu expressing β-glucosidase and cyanide hydratase for cyanogenic glycosides detoxification

  A recombinant Pichia strain GS115-Ch-Glu expressing both β-glucosidase and cyanide hydratase was constructed to remove cyanogenic glycosides in edible plants. The β-glucosidase could hydrolyze cyanogenic glycosides into cyanide and its gene (Glu) was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR). A cyanide hydratase could catalyze cyanide to formamide and its...

Author(s):   Chou-Fei Wu, Ai-Juan Feng, Xiao-Ming Xu, Si-Han Huang, Mao-Cheng Deng, Xin-Ning Zheng, Xiao-Ying Wu, Juan Peng, Jian-Ping Yuan and Jiang-Hai Wang      

March 2012

In silico identification of BIM-1 (2-methyl-1H-indol-3-yl) as a potential therapeutic agent against elevated protein kinase C beta associated diseases

  Protein kinase C beta is involved in apoptosis, transcription regulation, B-cell activation, regulation of the B-cell receptor signalosome and B-cell survival by regulating BCR-induced NF-kappa-B activation. Increased activation of PKC-βI along with overproduction of diacylglycerol (DAG) and vascular endothelial growth factor (VEGF) participate in pathogenesis of various aliments like asthma, cancer...

Author(s):   Umar Saeed#, Nayab Nawaz#, Yasir Waheed#, Nazia Chaudry#, Haleema Tariq Bhatti#, Samina Urooj#, Hafsa Waheed#, Muhammad Ashraf and Umar Hassan Khan Naizi.        

March 2012

Hematological and enzymatic responses of Nile tilapia Oreochromis niloticus during short and long term sublethal exposure to zinc

  Freshwater fish Oreochromis niloticus were exposed to sublethal concentrations of zinc for short and long terms. Responses of hematological parameters which include red blood cells count (RBC) and hemoglobin content (Hb) were investigated. Serumaspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities werealso determined. Two hundred and...

Author(s):   Younis, E. M., Abdel-Warith, A. A., and Al-Asgah, N. A.        

March 2012

Toxicological effects of arsenic exposure in a freshwater teleost fish, Channa punctatus

  High concentration of arsenic in groundwater in the north-eastern states of India has become a major cause of concern. Inorganic arsenic of geological origin is found in groundwater used as drinking-water in several parts of the world. Arsenic is used in various industries and agriculture and excessive arsenic finds its way into lakes and rivers. Since arsenic is a known human carcinogen,...

Author(s):   Sangeeta Das, Balagopalan Unni, Minakshi Bhattacharjee, Sawlang Borsingh Wann and Paruchuri Gangadhar Rao                                                               

March 2012

The influence of biopsy method on the survival rates of sexed and cryopreserved bovine embryos

  The purpose of this research was to find the most suitable embryo biopsy technique that would yield the highest pregnancy rates after the transfer of sexed frozen-thawed bovine embryos. Three methods of embryo biopsy (needle, blade or aspiration) were applied on 120 bovine embryos divided into 3 batches (n = 40). Subsequently, the embryos were frozen/thawed and transferred to synchronized recipients....

Author(s):   Mihai Cenariu, Pall Emoke and Groza Ioan        

March 2012

Three novel antioxidants from Cinnamomum plants

  In this study, we identified three novel antioxidants, subamolide C (1), subamolide E (2) and isokotomolide A (3) from the constitutes of Cinnamomum subavenium andCinnamomum kotoense. The radical scavenging properties and two more antioxidant activities of the three compounds were examined. All compounds presented effective 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging...

Author(s):   Kuo-Chen Cheng, Hsi-An Chen , Pei-Fang Wu, Woei-Ling Yang, Hui-Min Wang and Chung-Yi Chen      

March 2012

Antimicrobial susceptibilities of avian Escherichia coli isolates in Tabriz, Iran

  Colibacillosis is a poultry disease of economic importance in Iran and all around the world. The aim of this study is to test the antibiotic sensitivity of Escherichia colistrains which were isolated in Tabriz. A total of 100 E. coli strains isolated from aviancolibacillosis of 50 farms from 2008 to 2009 in Tabriz, were investigated for antimicrobial susceptibility. Antimicrobial resistance of...

Author(s):   Afshin Zakeri and Pedram Kashefi        

March 2012

The influence of cross-breeding Zlotnicka Spotted native breed sows with boars of Duroc (D) and Polish Large White (PLW) breeds on meat quality

  The aim of the study was to investigate the influence of cross-breeding of Zlotnicka Spotted sows with Duroc and Polish Large White boars on the quality of meat of crossbred fatteners. The investigation was done on 50 carcasses of fatteners from four genetic groups: Zlotnicka Spotted x Zlotnicka Spotted (n = 20), Zlotnicka Spotted x Polish Large White (n = 10), Zlotnicka Spotted x Duroc (n = 10) and Zlotnicka...

Author(s):   Karolina Szulc, Dariusz Lisiak, Eugenia GrzeÅ›kowiak and Sebastian Nowaczewski    

March 2012

Variation of the platelet indices of dromedary camel (Camelus dromedarius) with age, sex and breed

  The objective of this study was to determine reference intervals for platelet indices and hematological parameters in Saudi Arabian dromedary camels. Two hundred and twenty one apparently healthy dromedaries of Majahim (51 males and 31 females) and Maghatir (99 males and 40 males) breeds aged 1 - >10 yrs were investigated. Whole blood samples from the camels were analyzed for platelet...

Author(s):   Mansour F Hussein, AR Gar ElNabi, Adli N Aljarf, Riyadh S Aljumaah, Amel O Bakhiet and Mohammad A Alshaikh      

March 2012

Sexing bovine pre-implantation embryos using the polymerase chain reaction: A model for human embryo sexing

The paper aims to present a bovine model for human embryo sexing. Cows were super-ovulated, artificially inseminated and embryos were recovered 7 days later. Embryo biopsy was performed; DNA was extracted from blastomeres and amplified using bovine-specific and bovine-Y-chromosomespecific primers, followed by agarose gel electrophoresis. Embryos were then transferred to synchronized recipients and PCR...

Author(s): Cenariu M, Pall Emoke and Groza l