Monoclonal Antibodies Production Through Hybridoma Technology: A review
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Laboratory-made molecules known as monoclonal antibodies are designed to act as replacement antibodies and repair, improve, alter, or imitate the immune system's attack against unwanted cells. These antibodies identify specific binding sites, or epitopes, on a particular antigen. The protein sequence, antigen-binding site area, binding affinity for their targets, and downstream functional effects of mAbs are all similar....
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Cassava, a food security crop in Kenya, whose production is hampered by cassava brown streak disease (CBSD) and cassava mosaic disease (CMD). These challenges require urgent resistance breeding. Crossing of varieties in cassava is disadvantaged by poor and asynchronous flowering. This study aimed to induce and enhance flowering in CBSD immune and resistant clones with variable flowering characteristics. Two experiments...
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Studies suggested microorganisms in the rhizosphere aptitude in improving soils fertility without causing any environmental problem. Previous studies exhibited synergic interaction between these microorganisms and foliar bio-fertilizer as acceptable practice improving maize and soybean growth and yield in arid agro-climate in Korhogo, Northern Cote d’Ivoire. Herein, we developed R software fitting curve model, pointing to...
Bacillus thuringiensis: A Nature Package for Agriculture, Health and Industrial Application
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Bacillus thuringiensis (Bt) is a naturally occurring sporulating bacterium isolated worldwide from different ecosystems. It is packed with several Cry proteins that are toxic to different organisms. The presence of Cry toxins is the main factor conferring entomopathogenic properties to Bt which makes Bt different from other Bacilli. The Cry proteins are encoded by Cry genes, there are many Cry genes. Some single strains...
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Neem tree (Azadirachta indica A. juss) products, including seed, possess lots of scientific and economic benefits. Neem seeds contain adequate amount of azadirachtin, a triterpeniod compound, which can also be obtained from other parts of the plant. The compound acts as an insect repellent by inhibiting them from feeding and disrupting their increase reproduction. However, there may be sparse literature on secure human...
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Assessment of Genetic Diversity in Nigerian Local Chicken Population Based on Haematological Parameters was studied using one hundred and fifty (150) chickens comprising of fifty (50) Normal feathered, Frizzled feathered and Naked neck local birds were used for the study. The chickens were reared under deep litter system where the animalswere allowed to acclimatize for four (4) weeks.Males and females were reared...
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Introduction: Malaria is one of the most prevalent public health problems worldwide. Globally, nearly half the world’s population remains at risk of malaria transmitted by Anopheles mosquito vector. Chemical insecticides, which are toxic and cause environmental deprivation, have been used to control the vector. So,safe environmentally friendly and effective alternative method are need to control malaria vectors. ...
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The search for more safe and nutritive natural sweeteners as an alternative to commercial sweeteners, which are implicated in obesity and diabetes mellitus has led to the utilization of the African locust bean (Parkia biglobosa) pulp which is rich in dietary fibres, soluble sugars and vitamins. Mango peels contain antioxidants, fiber and a source of Nutraceuticals. The mixture of the pulp and mango peel were blended and...
Single nucleotide polymorphisms (SNPs) and their Genotypes in the three Breeds of Sheep
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Single nucleotide polymorphisms (SNPs) and their Genotypes in the three Breeds of Sheep was conducted with ninety (90) sheep, thirty from each breed (30 Balami, 30 Yankasa and 30 Uda) sheep were used for the study. Phenotypic characterization, blood sample collection, DNA Extraction, Polymerase Chain Reaction (PCR) and Single-Strand Conformational Polymorphism Analysis (SSCPA), Agarose gel electrophoresis, Sequencing of...
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Escherichia coli (E. coli) and Salmonella enterica (S. enterica) are Gram-negative bacteria commonly found in the gastrointestinal tract of animals and can cause illness in humans, particularly through contaminated meat products and their handling. Genetic identification techniques offer rapid and precise methods for characterizing these bacteria and revealing their genetic diversity. This study aimed to investigate the...
A Relationship between Soil bacterial Community and Ginseng Rusty Root Disease
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Ginseng rusty root disease (GRRD) is one of the major diseases of ginseng, which disease is leading to a severe decline in the quality and yield of ginseng. Soil bacterial communities’ long-term survival in soil, and it plays an important role in the growth of ginseng health. To clarify the relationship between soil bacterial community and GRRD, soil properties, bacterial community structure and diversity from eight sites...
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In Ikwoka, Southeast Nigeria Hemisus guttatus and Milvus migrans parasitus are good comparatively bio indicators due to non migration and occupation of different terrestrial environments during seasonal migrations respectively. Hemisus guttatus is rare while Milvus migrans parasitus is seasonally frequent. This study is to establish possible cause of Hemisus guttatus rare status between parasites and other environmental...
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Sesame (Sesamum indicum L.) is a significant oilseed crop that is cultivated in various regions across the globe. Its seed is well known for its high oil content (50-60%), thus earning it the title of the supreme oilseed. Nigeria, being among the top 10 largest producers of sesame, possesses immense potential to enhance production for the purpose of promoting exports. Sesame exhibits extensive genetic diversity, which can...
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A study was conducted to determine the genetic variability and patterns of diversity of finger millet (Eleusine coracana) accessions using six ISSR molecular markers. The study consisted of 72 finger millet accessions with variable seed coat colors collected from two zones of the Tigray region. The markers generated 45 loci, 30 of which were polymorphic and 15 of which were monomorphic, for an average of 7.5 loci per...
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In my research, the results of the physical-chemical analysis and sensory analysis of wine production from local and otheri valuable technical grape varieties cultivated in the diverse and rich soil-climatic conditions of our country and the produced wines were reflected. Samples were taken from the produced products, the amount of sugar content, acidity and other important indicators were determined by modern methods and...
Brewing 4.0: Unleashing the Power of Industrial Internet of Things for Smart and Sustainable Brewing
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The rapid evolution of the Internet of Things (IoT) has given rise to the Industrial Internet of Things (IIoT) otherwise known as the 4th Industrial Revolution. Embracing IIoT in the brewing industry, also known as Brewing 4.0, promises revolutionary advancements in brewery operations. Breweries like Ab InBev and Sugar Creek are advanced levels of implementation of IIoT. The implementation of which has decreased process...
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Septoria leaf blotch is one of the most devastating diseases of wheat worldwide. Understanding its genetic diversity and population structure is paramount importance in designing and sustainable management strategies to control the disease. Therefore, the present study was designed to detect and determine the genetic diversity and population structure of Z. tritici isolates collected from northern Ethiopia: Amhara and...
The Antioxidant, Protective, and Nutritional Roles of Folic Acid “Vitamin M”
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Folic acid is a water-soluble vitamin that comes after the vitamin B complex and is one of the compounds that have antioxidant activity and protective against some diseases caused by harmful oxidation. Recently, through studies and scientific research conducted on folic acid, the extreme importance of this vitamin has emerged after cases of deformity occurred in children due to a lack of nutrition from sources containing...
NEEM (OIL) AND CROP DEFENCE: (A desire for the future)
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The mission of agriculture is to improve food production to satisfy the needs of the developing global populace, without damaging the surroundings. In Present day agricultural practices, the control of pests is regularly performed via the excessive use of agrochemicals that can bring about environmental pollutants and the development of resistant pests. In this context, biopesticides can offer a better opportunity for...
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Auricularia species is a jelly- like edible fungus usually with mild flavour. It is also known as wood ear mushroom because of its ear-shaped morphology. Nigeria is blessed with many species of these edible mushrooms; however, not much research effort has been given to the cultivation of Auricularia species. Wild species of Auricularia were collected from different locations within southern Nigeria. Pure cultures of these...
ADSORPTION OF CHROMIUM (VI) AND LEAD (II) IN SYNTHETIC SOLUTIONS USING TAMARINDUS INDICA FRUIT PEEL.
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Adsorption allows minimizing the generation of toxic waste and the recovery of the metal. The objective of the work was to study the bioadsorption of Cr (VI) and Pb (II) using the dry peel of Tamarindus indica. We worked at different pH values and concentration levels. The determination of the chemical-physical parameters was carried out at the Empress Geominera Oriente. Adsorption isotherms were performed using the...
AI-Metaverse Bioinformatics: Potential future direction
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Bioinformatics has been around since the 1960s, while online molecular databases that handle data generated by disciplines in the life sciences have existed since the 1990s. This Perspective presents a forward-looking approach that amalgamates bioinformatics with new and potential fields such as generative artificial intelligence and metaverse that can catapult bioinformatics into user-friendly 3D virtual worlds with...
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The specific objectives of this research were (i) to develop broad-scale bioinformatics data from three lactic acid bacteria (LAB) strains - S. thermophilus KLDS 3.1003, SMQ-301, and LMD-9 genomes, (ii) to mine the relevant genes that confer probiotic and industrial properties to the strains, and (iii) show a more precise correlation between reported in vitro and in vivo properties of these strains and their genome...
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Meat is highly nutritious, being a good source of various nutrients especially protein, fat and vitamins. Its composition is ideal for the growth of a wide array of spoilage bacteria. Food-borne infections outbreaks affect both developing and developed nations. The infections caused by emerging pathogens like Enterohaemorrhagic E. coli 0157, Listeria monocytogenes, Vibrio vulnificus, Campylobacter specis and...
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A large part of transcripts is non-coding, which is transcribed from junk DNA; long non-coding RNAs and micro-RNAs regulate the expression levels of mRNAs. For the first time, we identified lncRNAs and miRNAs with their regulatory role in the disease tolerance of Theobroma cacao. In this study, about 2616 lncRNAs and 153 miRNAs were identified from 10 RNA-seq data representing healthy and witches’ broom diseased tissues of...
Evaluation of Artificial Insemination Delivery Services to Smallholder Dairy Farmers in Ethiopia
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The study was carried out in dairy production potential areas of Oromiya, Amhara and Sothern Nations Nationalities People’s Region (SNNPR) with the objectives of evaluating artificial insemination delivery service on small holder dairy farmers, identify major constraints of artificial insemination delivery service and identify ways of alleviating problems related to artificial insemination delivery service. The study...
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Hexavalent Cr is most toxic metal and hazardous compound worldwide. It is also considered to be cellular oxidizing pollutant causing damage, mutagenic and hematopoietic effects to living systems. Present work is focused on synthesis of zero valent iron nano particles (Fe-NPs) using Bacillus subtilis SHB 13, Achromobacter xylosoxidans SHB 204 and Surfactin and their application for Cr (VI) detoxification (100 ppm) in...
Quantitative Phytochemicals and Antioxidant Potential of Adansonia digitata leaves and stem extract
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The objective of this study was to examine the phytochemical properties of the baobab tree (Adansonia digitata) stems and leaves. The dried powder of leaves and stem extract were analyzed for the presence or absence of different phytochemicals, flavonoids, terpenoids, saponins, tannins, alkaloids, etc. The quantitative analysis revealed that the leaves and stem of A. digitata are rich in total phenols, alkaloids,...
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Dekoko is a marginalized, but economically important and endemic cool - season legume crop in Ethiopia. However, the molecular characterization of this crop has not been carried out regardless of its importance as source of protein for the rural poor in Ethiopia. This study is therefore aimed to investigate the genetic diversity and population structure of 83 Dekoko accessions collected from north Ethiopia. Three plants...
An overview of proteolytic enzymes and determination of protein content in the proteinous sample
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The world environment is changing by the socio economic factors. Most of this occurs by using chemical in industries. Digestive enzymes plays a great role in breakdown of macromolecules into micro molecules Protease also called proteinase or peptidase is a digestive enzyme that categorized under proteolytic enzymes and replaces these chemicals in industry. Protease categorized into different classes based on different...
Fermented Bamboo Shoot Health Benefits and Applications
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The term "bamboo" conjures up images of "a hollow stick," and it aids more health benefits. Mainly the tribes of northeast India ferment these nutritious bamboo shoots. Fermentation is a biotechnological process that has been used to preserve food for centuries. Fermented bamboo shoots caters a home and benefits for a variety of microorganisms. To highlight a few benefits these magical shoots are probiotic, anti-oxidant,...
Phytochemical, antioxidant and anti-proliferative activity of Epipremnum aureum
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Objective: The study was primarily carried out to assess the phytochemical contents present in the leaf of E. aureum. Herein, biochemical characterization vis-à-vis antioxidant potential and antiproliferative activity on cancer cell lines is experimented too. Methods: Healthy leaves of E. aureum after authentication were evaluated in vitro for phytochemical, antioxidant and antiproliferative activity. Various non-enzymatic...
Comparative physiological studies of parental and fusant protoplasts culture of Volvariella volvacea
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Very little information is available in the physiology, biochemistry and molecular biology of Volvariella volvacea. The current study was carried out to understand the effect of different macro- and microelements and vitamins on the mycelial growth and yield of protein by the mycelia of parental and fusant protoplasts strain of V. volvacea. The method adopted in this experiment was to study the additive and deficient...
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Application of DNA fingerprint is the method which permits documentation of genetic material with high accuracy and permanency as it is not predisposed by environment, epistatic interactions and other factors. Seed quality and purity are the important key aspects to be maintained in seed multiplication and production to enhance yield and productivity. The aim of this study was to assess genetic purity of yellow bean...
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Background: Hypertension is a common debilitating illness among people in both developed and developing countries. This study investigated the effect and possible mechanism of the antihypertensive activity of lycopene-rich extract of Solanum lycopersicon (LRESL) on Wistar rats. Methods: Sixty (60) hypertensive Wistar rats were divided into seven experimental groups viz: Group A served as a normotensive group and received...
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Lignocellulosic biomass is applauded to be the cheapest resource for second generation biofuels. However, the degradation of lignocellulosic biomass is quiet challenging due to the complexity in structure. The study investigated the saccharification potential of in-house enzymes produced from a white-rot basidiomycete strain, Polyporus ciliatus CBS 366.74 on a lignocellulosic biomass such as pretreated corn stover. The...
Genetic characterisation of Colophospermum mopane and its ecotypes using RAPD analysis
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Colophospermum mopane and its ecotypes are currently recognized on morphological and physiological characters. To add to the suite of taxonomic characters, the genetic variability of C. mopane (sensu stricto) and its ecotypes were investigated using Random Amplified Polymorphic DNA. DNA was extracted from young and mature leaves using the CTAB method. Twenty random primers were tested for their suitability and...
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AFLP markers were used for evaluating genetic diversity, population substructure, species relationships, and gene flow among eighty-five accessions belonging to the mungo-radiata group of section Ceratotropis of subgenus Ceratotropis of genus Vigna. Twelve preselected AFLP primers generated 1869 polymorphic amplification products. The number of fragments for each primer pair ranged from 44 to 296, showing 100%...
Multi-location Study of Six-Rowed Barley Lines for Malt Quality
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In Ethiopia, both two- and six-rowed barleys can grow in the highlands. However, the country is not self-sufficient in barley malt, and it depends on imports from different overseas countries. Little effort has been made in two-rowed malt barley variety release and production to overcome the low malt barley supply. So far, the lack of six-rowed barley varieties with good malt quality is a gap in malt barley research in...
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This study investigates the pretreatments and drying method effects on white yam’s physicochemical, microbial, and sensory qualities. The fresh tubers were washed, (equally) uniformly sliced, pretreated, dried, and processed into flours. The flours were evaluated for physicochemical, microbial, and sensory properties using standard methods. The results showed that all physicochemical parameters examined were significantly (p
A review of traditional fermented foods and beverages of the Democratic Republic of Congo
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Several traditional fermented foods and beverages are produced at the household level in the Democratic Republic of Congo. These include fermented cereal-based porridges from sorghum or eleusine ( musululu ), from corn (mandrakwa and Munkoyo); fermented cereal-based foods (bakinde), fermented milk products (mashanza and kivuguto), fermented root-based foods (kwanga, lobe, tshomba tshia kabiola, tshomba tsha mbenzu,...
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Development of the multiple antibiotic resistance is the major cause of clinical infections world wide and has been increasing. This study determined the pattern of bacterial multiple antibiotic resistance in hospital sewage samples obtained from Marathwada region of India. The present study had used 48 isolates of bacteria obtained from 6 different locations of Aurangabad, Marathwada India. Antibiotic sensitivity test...
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The water-uptake pattern of Tiger-nut (Cypecus esculentus) yellow variety was determined at 30, 35, 40, 45 and 50oC during soaking for 0 - 96 hours. Also, the microbial load of the soak water at the point of full turgidity of soaked tiger nut at temperatures of 30, 35, 40, 45 and 50oC was were also determined. The hydration curves obtained showed shows that most of the moisture uptake took place within the first 24 hours,...
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Background: Little research effort is directed towards addressing the controversies surrounding the effects on caffeine on humans. Its increasing consumption and industrial application is why researches need to be geared up in this area. This study investigated the hepatoprotective effect of Myristica fragrans following concomitant oral administration of caffeine using histopathological, histochemical and biochemical...
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Sorghum [Sorghum bicolor (L.) Moench], a cereal crop of family Poaceae, is believed to be originated in Ethiopia and Sudan. It is an important food security crop mainly in semi-arid and tropical parts of the African countries. Although many morphological and molecular diversity studies reveal the existence of genetic variations with sorghum populations, their distribution within basic races were not considered. Hence, the...
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Jatropha is a drought-tolerant plant producing seed oil for the biodiesel. Limitation to the development of jatropha is unavailability of high-yielding varieties and efficient in vitro regeneration system which is required for micropropagation. In this study, in vitro regeneration system from jatropha juvenile cotyledon was established. Firstly, concentrations of hormones in the MS medium were optimized and it was found...
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Biological and nutritional stressors can compromise the welfare of birds. These are due to changes in the normal levels of biochemical and physiological parameters of the birds which can be measured at different loci to determine the severity of the stresses. This study evaluated the haematological and biochemical traits as stress indicators in three genetic strains of broilers (Arbor Acre, Cobb-500, Marshall) reared at...
Survival rate of donkey foals: status quo and improvement methods
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The industrial improvement of donkey-hide gelatin and the development of related products, such as donkey meat, donkey milk, and donkey-hide gelatin cake, drive the upstream donkey breeding industry. However, the shortage of donkey germplasm resources seriously restricts the further development of the donkey industry. Under the biological limitations of long breeding cycle, slow breeding, low conception rate, high abortion...
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The present research was undertaken to identify the bioactive compounds present in two varieties of Mucuna pruriens var utilis: (IIHR Selection 3 and Arka Dhanvantari) and their pharmaceutical importance evaluated through phytochemical and in-silico screening. The phytochemical screening of the leaf and seed extracts revealed the presence of alkaloids, tannins, flavonoids, phenols, proteins, terpenoids, reducing sugars,...
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Hormesis is a biphasic dose (concentration)-response phenomenon characterized by low dose stimulatory and high dose inhibitory effects exerted by stressors on living organisms. Recently, there has been increased interest in the phenomenon and statistical models for its exploration in toxicological studies. The bilogistic model of Beckon et al. (2005) is one of such models for modeling biphasic dose-response relationships...
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