African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12485

Table of Content: March 2005; 4(2)

March 2005

Role of bioinoculants in development of salt-tolerance of Vicia faba plants under salinity stress

  Through biological inoculation technology, the bacterial-mycorrhizal-legume tripartite symbiosis in saline conditions was documented and the effects of dual inoculation with Azospirillum brasilense (NFB) and Arbuscular mycorrhizal (Am) fungus Glomus clarum on the host plants (Vicia faba) in pot cultures were investigated at five NaCl levels (0.0 – 6.0 dSm-1) in irrigating water. Am...

Author(s): G. H. Rabie and A. M. Almadini

March 2005

Response of Musa species to macro-propagation. I: Genetic and initiation media effects on number, quality and survival of plantlets at prenursery and early nursery stages

  Ricehull and sawdust were evaluated as Musa sucker plantlet initiation media using five genotypes as test plants. Sword-sucker-corms whose apical dominance was physically destroyed were planted and evaluated for plantlet production during a period of about five months. The number, quality and pattern of plantlets produced and their survival were studied. Results showed that initiation media had...

Author(s): Baiyeri, K. P. and Aba, S. C.

March 2005

Response of Musa species to macro-propagation. II: The effects of genotype, initiation and weaning media on sucker growth and quality in the nursery

  Sucker plantlets of five Musa genotypes were derived from sword-sucker-corms using ricehull and sawdust as initiation media. Plantlets initiated were transferred to three weaning/rooting media formulated with ricehull (RH), sawdust (SD) and poultry manure (PM). Weaning media and genotypes had significant (P<0.05) effects on most of the sucker plantlet growth parameters studied. There was variable...

Author(s): Baiyeri K. P.

March 2005

Anther culture response in indica rice and variations in major agronomic characters among the androclones of a scented cultivar, Karnal local

  Effect of organic adjuvants, synthetic plant hormones and diverse carbon sources in influencing anther culture response in five indica rice genotypes (IR 72, Mansarovar, Tarori Basmati, Pusa Basmati and Karnal local 95) were assessed. Androgenic callus induction as well as green plantlet regeneration was more when callus was induced on N6 fortified with 100 mg L-1 YE. However, coconut water (5...

Author(s): Bidhan Roy and Asit B. Mandal

March 2005

Photoperiodism in Digitaria exilis (Kipp) Stapf accessions

  Three varieties of Digitaria exilis namely, white mbulus, jakashale and red dapys were grown in planting chamber and the effect of photoperiod on flowering was investigated. The photoperiods were eleven, twelve and thirteen hours. Highly significant differences (p<0.01) were recorded for the number of days to flowering as affected by photoperiods. The interaction between the photoperiods and...

Author(s): A.A. Aliero and J.A. Morakinyo

March 2005

Somatic embryogenesis in date palm (Phoenix dactylifera L.) from apical meristem tissues from ‘zebia’ and ‘loko’ landraces

  The shoot apical meristem from young suckers were used as sources of explants for initiation of culture using MS basal medium which contained 2,4-D. This was incubated at 27oC in the dark. Callogenesis was observed as early as the second subculture. Continuous subculture of the callus in the establishment medium at about the third subculture from calls production, resulted in somatic embryo formation. The...

Author(s): Chukwuemeka R. Eke, Peter Akomeah and Omorefe Asemota

March 2005

Quantitative trait loci for head-bug resistance in sorghum

  QTLs were mapped in F2 progeny derived from a cross between the head-bug resistant sorghum cultivar Malisor 84-7 and susceptible S 34. The phenotypic evaluation was conducted in Mali. The mapped population consisted of 217 F2 plants, with 345 homologous and heterologous RFLP probes and 49 microsatellite markers tested. Eighty-one RFLP markers revealed polymorphism between the two parents, and 14...

Author(s): M. Deu, A. Ratnadass, M. A. Hamada, J.L. Noyer, M. Diabate† & J. Chantereau

March 2005

Effect of cultural conditions on xylanase production by Streptomyces sp. (strain Ib 24D) and its potential to utilize tomato pomace

  The purpose of this study was to determine the effect of some cultural conditions on the xylanase enzyme production by Streptomyces sp. (strain Ib 24D) and to investigate its potential to produce xylanase utilizing tomato pomace as a substrate. Xylanase activity was detected using the dinitrosalicylic acid assay method. The crude enzyme was maximally active at pH 6.5 and 60°C. The maximal enzyme...

Author(s): Rifaat Rawashdeh, Ismail Saadoun and Amjad Mahasneh

March 2005

Isolation and Identification of a Brevibacterium linens strain degrading p-nitrophenol

  A bacterium was isolated from garden soil in basal salts medium containing p-nitrophenol (PNP). Subsequent subcultures in agar, nutrient agar plates and agar slants by streaking led to isolation of pure colonies. The pure culture could degrade up to 300 mg/L PNP in presence of yeast extract. It was Gram positive rods, mostly single, catalase-positive, hydrolyzing strach and casein but not urea. Gelatin...

Author(s): Debananda Ningthoujam

March 2005

Activity of essential oil and phenolic acid extracts of pepperfruit (Dennetia tripetala G. Barker; Anonaceae) against some food-borne microorganisms

  A previous report showed that the essential oil and phenolic acid extracts of pepperfruit (Dennetia tripetala) inhibited the growth of tomato-rot fungi. The study was subsequently extended to other food-borne microorganisms (Staphylococcus aureus, Salmonella sp. Pseudomonas aeruginosa., Proteus sp., Escherichia coli., Enterococcus faecalis., Serratia sp., Bacillus sp., Clostridium sp., Penicillium sp.,...

Author(s): B. O. Ejechi and  D. E. Akpomedaye

March 2005

Increase of nisin production by Lactococcus lactis in different media

  Nisin production related to the growth conditions of Lactococcus lactis subsp. lactis ATCC 11454, the effects of various media components and concomitant release of nisin into the media, were studied through transfers (five times). Nisin production was assayed by agar diffusion using Lactobacillus sake ATCC 15521 as the sensitive test organism. The expression of nisin was strongly...

Author(s): Angela Faustino Jozala, Letícia Célia de Lencastre Novaes, Olivia Cholewa, Dante Moraes, Thereza Christina Vessoni Penna

March 2005

Studies on the effect of abattior and microbial quality of Aba river in Nigeria

  Levels of lead, iron, zinc, copper, arsenic, cobalt, chromium, manganese, mercury and cadmium, as well as the microbial profile were determined in water samples from Aba River. Physico-chemical examinations revealed that manganese (0.03 mg/l), zinc (4.81 mg/l) and copper (0.19 mg/l) were below the maximum allowable levels set by the United States Environment Protection Agency (USEPA), while lead (0.064 mg/l),...

Author(s): O.U. Ezeronye and A.O. Ubalua

March 2005

Methods of preparation and nutritional evaluation of dishes consumed in a malaria endemic zone in Cameroon (Ngali II)

  This study deals with the description of the methods of preparation and determination of the nutritional potential of dishes consumed by Cameroonians living in a rural area, which is a malaria endemic zone, called Ngali II. The dishes consumed are prepared from leguminous seeds, seeds of the Cucurbitaceae family (egusi seeds), green leafy vegetables, tubers, cereals unripe bananas and plantains. The contents...

Author(s): Roger Ponka, Elie Fokou,   Rose Leke, Martin Fotso, Jacob Souopgui, Mercy Achu Bih And Félicité Mbiapo Tchouanguep

March 2005

Temperature effect on dextransucrase production by Leuconostoc mesenteroides FT 045 B isolated from Alcohol and Sugar Mill Plant

  Temperature (23 to 31°C) and sucrose concentration (3 and 4%) effects on dextransucrase production by Leuconostoc mesenteroides NRRL B 512 (F) andLeuconostoc mesenteroides FT 045 B were studied. The conditions in all fermentations were:  total reaction volume 2 L, 132 rev. min-1, 0.5 vvm and pH 6.0. The optimum temperature for enzyme yield for strain NRRL B 512 (F) was 23°C, where...

Author(s): Mariana Cortezi, Rubens Monti and Jonas Contiero

March 2005

Identification of metallo-b-lactamase from a clinical isolate at Saint Camillle Hospital Center of Ouagadougou/ Burkina Faso

A metallo-b-lactamase was identified from a clinical sample. The host bacteria was identified to be Chryseobacterium Indologenes. This preliminary characterization of the enzyme is reported here.   Key words: b-lactamase , metallo-b-lactamase,Chyseobacterium Indologenes, Ouagadougou.

Author(s): Boukaré Zeba, Pere Jacques Simporé,  Odile G. Nacoulma,  Jean-Marie Frère

March 2005

Studies on the extraction and characterization of thermostable a-amylase from pericarp of Borassus indica

Thermostable a-amylase was extracted and characterized from the fruits (pericarp) of Borassus indica.  Analysis on the influence of various physico-chemical parameters on the extracted enzyme revealed a Vmax of 0.793 and a Km of 0.022. The optimum temperature was found to be 370C at pH 4.5. The stability studies on enzyme activity envisaged that the enzyme is stable up to 800C and retained its activity...

Author(s): M. Srinivasa Rao, N.S. Reddy, G. Venkateswara Rao and K.R.S. Sambasiva Rao