African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 425

Full Length Research Paper

Optimization of metalloprotease production by Eupenicillium javanicum in both solid state and submerged bioprocesses

Youssef Ali Abou Hamin Neto1, Cristina Maria de Souza Motta2 and Hamilton Cabral1*
  1Enzyme Technology Laboratory, Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences, Ribeirão Preto-USP, Ribeirão Preto, Brazil. 2Micology Department, Federal University of Pernambuco, Recife, Brazil.
Email: [email protected]

  •  Accepted: 30 July 2013
  •  Published: 30 September 2013



Proteases are enzymes that have great importance in industries and can be produced through bioprocess by microorganisms. Changes in parameters can influence the production rate of these enzymes. The current study evaluated the influence of these parameters on the production of proteases by the fungus Eupenicillium javanicum under both solid state bioprocess (SSB) and submerged bioprocess (SmB), and determined the partial biochemical characterization of the crude enzymatic extract. Our studies have indicated that the best conditions for the production of proteases under SSB were a 10% albumin, 90% wheat bran incubated at 30°C. Under SmB the optimal conditions were medium supplemented with 0.25% casein, medium pH of 5.0 and incubation at 30°C. The biochemical characterization showed that the enzyme is a metalloprotease that presented collagenolytic activity, with optimal pH and temperature of 5.5 and 60°C, respectively.


Key words: Metalloprotease, Eupenicillium javanicum, bioprocesses, collagenolytic activity, biochemical characterization