African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 425

Full Length Research Paper

The protective role of oral consumption of N-acetyl cysteine during a single session of exhaustive exercise in untrained subjects

Iranpour D
  • Iranpour D
  • Department of Cardiology, Faculty of medicine, Bushehr University of Medical Sciences, Moallem Street, Bushehr, Iran.
  • Google Scholar
Movahed ALI
  • Movahed ALI
  • Department of Clinical Biochemistry, Faculty of Medicine, Bushehr University of Medical Sciences, Moallem Street, Bushehr, Iran.
  • Google Scholar
Goudarzi A
  • Goudarzi A
  • Faculty of Human Sciences, Islamic Azad University, Bushehr Unit, Bushehr, Iran.
  • Google Scholar
Tarverdizadeh Bahman
  • Tarverdizadeh Bahman
  • Faculty of Human Sciences, Islamic Azad University, Bushehr Unit, Bushehr, Iran.
  • Google Scholar
Akbarzadeh Samad
  • Akbarzadeh Samad
  • Department of Clinical Biochemistry, Faculty of Medicine, Bushehr University of Medical Sciences, Moallem Street, Bushehr, Iran.
  • Google Scholar
Motamed Niloufar
  • Motamed Niloufar
  • Department of Community Medicine, Faculty of Medicine, Bushehr University of Medical Sciences, Moallem Street, Bushehr, Iran.
  • Google Scholar
Pakdel Farhad
  • Pakdel Farhad
  • Department of English, Faculty of Paramedical Sciences, Shiraz University of medical Sciences, Shiraz, Iran.
  • Google Scholar
Hagian Ali Akbar
  • Hagian Ali Akbar
  • Department of Pharmacology, School of Allied Medical sciences, Tehran University of Medical Sciences, Tehran, Iran.
  • Google Scholar
Seyedian Ramin
  • Seyedian Ramin
  • Department of Pharmachology, Faculty of Medicine, Bushehr University of Medical Sciences, Moallem Street, Bushehr, Iran.
  • Google Scholar
Vahdi Saeid
  • Vahdi Saeid
  • Department of Pharmacology, School of Allied Medical sciences, Tehran University of Medical Sciences, Tehran, Iran.
  • Google Scholar
Tahmasebi Rahim
  • Tahmasebi Rahim
  • Department of Biostatistics, Faculty of Health, Bushehr University of Medical Sciences, Bushehr, Iran.
  • Google Scholar


  •  Accepted: 23 December 2013
  •  Published: 31 January 2014

Abstract

The aim of the current study was to verify the effect of N-acetyl cysteine (NAC) supplementation on markers of oxidative stress and inflammatory response during a single session of exhaustive exercise. In a randomized placebo-controlled double-blind clinical trial, thirty healthy, untrained young males and females with a mean age of 21.33±2.39 years, weight of 59.63± 9.24 kg and height of 166.20±10.15 cm were selected and divided into 2 groups. Before starting the supplementation, blood samples were collected from all the participants. The next blood samples were collected just before the exercise started, immediately after the exhaustive exercise and after one hour of rest. Malondialdehyde (MDA), total antioxidative capacity (TAC), CRP, BMI and Vo2 max were determined. A significant increase was observed in MDA and CRP levels during the experiment in the placebo group. But in the treated group, the concentrations remained the same throughout the experiment. The TAC levels were significantly raised in the samples collected after NAC supplementation as compared to the placebo group. No changes were observed in the time to fatigue. Results of the current study suggest that oral consumption of n-acetyl cysteine for 24 h before a single bout of an exhaustive physical exercise could significantly reduce the harmful effects of oxidative stress.

Key words: N-acetyl cysteine, oxidative stress, exhaustive exercise, reactive oxygen species, time to fatigue.

Abbreviation

BMI, body mass index; CRP, C-reactive protein; MDA, malondialdehyde; CK, creatine kinase; TAC, total antioxidative capacity; NAC, N-acetyl cysteine;  Vo2 max, maximal oxygen uptake; TBARs, thio-barbituric acid reactive substance; GPx, glutathione peroxidase; GR, glutathione reductase; GSH, reduced glutathione.