African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 425

Full Length Research Paper

Screening of antioxidant activity, total phenolics and gas chromatograph and mass spectrometer (GC-MS) study of delonix regia

P. Maria jancy Rani1*, P. S. M. Kannan2 and S. Kumaravel3
1Department of Chemistry, Dravidian University, Srinivasavanam, Chittoor District, Kuppam-517425, Andhra Pradesh, India. 2PRIST University, Vallam, Thanjavur-613 403, Tamil Nadu, India. 3Food Testing Laboratory, Indian Institute of Crop Processing Technology, Pudukkottai Road, Thanjavur-613 005. Tamil Nadu, India.
Email: [email protected]

  •  Accepted: 07 October 2011
  •  Published: 31 October 2011


The present study was carried out for identification of the phytochemicals present in the Delonix regia leaves and also evaluates the total phenols, total flavonoids and antioxidant activity of the leaf extract. Total phenols were carried out by Folin Ciocalteu method and the phenolic content was 16.00 mg/100 g of gallic acid equivalent (GE). Antioxidant activity was evaluated by 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) method and the leaves of D. regia showed 10.73 mg/100 g of ascorbic acid equivalent antioxidant capacity (AEAC). The gas chromatograph and mass spectrometer (GC-MS) study was also carried out and it showed the presence of phytochemicals like phytol (RT: 15.49), Cumarin 7, 8-dihyddro-7-hydroxy-6-methoxy-8-oxo (RT: 15.92), Squalene (RT: 25.41) and Vitamine (RT: 29.97).


Key words: Total phenols, total flavonoids, antioxidant activity, 2,2-Diphenyl-1-Picrylhydrazyl (DPPH), gas chromatograph and mass spectrometer  (GC-MS), delonix regia.


GE, Gallic acid equivalent; DPPH, 2,2-diphenyl-1-picrylhydrazyl; AEAC, acid equivalent antioxidant capacity; GC-MS, gas chromatograph and mass spectrometer; UV,ultraviolet; NIST, National Institute Standard and Technology; O.D, optical density.