African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 425

Full Length Research Paper

Effects of n-butanol fraction of Gongronema latifolium leave extract on some biochemical parameters in CCl4- induced oxidative damage in Wistar albino rats

Okpala, J. C
  • Okpala, J. C
  • Department of Biochemistry, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
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Sani, I
  • Sani, I
  • Department of Biochemistry, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
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Abdullahi, R
  • Abdullahi, R
  • Department of Biochemistry, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
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Ifedilichukwu, H. N
  • Ifedilichukwu, H. N
  • Department of Medical Biotechnology, National Biotechnology Development Agency, Abuja, Nigeria.
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Igwe, J. C
  • Igwe, J. C
  • Department of Medical Biotechnology, National Biotechnology Development Agency, Abuja, Nigeria.
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  •  Accepted: 16 January 2014
  •  Published: 28 February 2014

Abstract

Effects of n-butanol fraction of Gongronema latifolium leave extract on some biochemical parameters in carbon tetrachloride-induced oxidative damage in Wistar albino rats were assessed. Fifty-four (54) Wister albino rats were divided into treatment group and LD50 groups. Group A (normal control) was given feed and water, Group B (vehicle control) was injected with olive oil intraperitoneally, while the rest groups (C, D, E, F and G) were injected intraperitoneally with a single dose of CCl4 (148 mg/kg) as a 1:1 (v/v) solution in olive oil and all the animals were fasted for 36 h. This was repeated once every week for a period of four (4) weeks. At the end of 28 days of treatment, liver marker enzymes studies showed that there was significant (p<0.05) increase in the serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP)and bilirubin concentrations in CCl4-induced control group when compared with the normal control and induced treated groups but there was no significant (p>0.05) difference of these liver marker enzymes and bilirubin levels between the normal control and induced treated groups. Kidney function studies showed that there was significant (p<0.05) increase in creatinine and urea concentrations of CCl4-induced control group when compared with the normal control and induced treated groups but there was no significant (p>0.05) difference between the normal control and induced treated groups. Also, the kidney and liver antioxidant study revealed significant (p<0.05) decrease in superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities as well as an increase in MDA concentration in CCl4-induced control rats when compared with the normal control rats. Histological section of the organs shows that the levels of hepatic and renal damage were higher in CCl4-induction control rats when compared with the normal control and induced treated groups. These findings suggest that n-butanol fraction of methanolic leaves extract of G. latifolium may have anti-hepatotoxic, anti-nephrotoxic and antioxidative effects against CCl4-induced liver and kidney damage in albino rats.

Key words: Gongronema latifolium, antioxidant, CCl4, liver, kidney.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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