African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 425

Full Length Research Paper

Screening of antioxidant activity, total phenolics and gas chromatography-mass spectrophotometer (GC-MS) study of ethanolic extract of Aporosa lindleyana Baill

S. Ramakrishnan1* and R. Venkataraman2
1Department of Biotechnology, Sri Paramakalyani College, Alwarkurichi – 627 412, Tirunelveli District, Tamil Nadu, India. 2Post graduate and Research Department of Chemistry, Sri Paramakalyani College, Alwarkurichi – 627 412, Tirunelveli District, Tamil Nadu, India.
Email: [email protected]

  •  Accepted: 02 November 2011
  •  Published: 31 December 2011


The present study was carried out for the identification of phytochemicals present in the roots ofAporosa lindleyana and also to evaluate the total phenol, total flavonoids and antioxidant activity. Total phenol was carried out by Folin Ciocalteu method and the phenolic content was 31.20 mg/100 g of gallic acid equivalent (GE) and the flavonoid content was 203.10±0.9. Antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and the roots of A. lindleyana showed 19.91 mg/100 g of ascorbic acid equivalent antioxidant capacity (AEAC). The gas chromatography-mass spectrophotometer (GC-MS) study was also carried out and it showed the presence of phytochemicals like 1,2-benzenedicarboxylic acid, diphenyl ester (RT: 11.97), pthalic acid, bis (7 methylocytyl) ester (RT: 20.86) and squalene (RT: 24.70).


Key words: Aporosa lindleyana, antioxidant, flavonoids, gas chromatography-mass spectrophotometer (GC-MS), 2,2-diphenyl-1-picrylhydrazyl (DPPH).