African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 425

Full Length Research Paper

Production of tannase and gallic acid by Aspergillus tamarii in submerged and solid state cultures

Andrea Miura da Costa1, Cristina Giatti Marques de Souza2, Adelar Bracht2, Marina Kimiko Kadowaki3, Aline Cristine da Silva de Souza2, Roselene Ferreira Oliveira2 and Rosane Marina Peralta2*
1State University of Santa Cruz, Ilhéus, BA, Brazil. 2State University of Maringá, Maringá, PR, Brazil. 3State University of West Paraná, Cascavel, PR, Brazil.
Email: [email protected], [email protected].

  •  Accepted: 17 September 2013
  •  Published: 30 November 2013


The hydrolytic enzyme, tannase and the antioxidant phenolic compound, gallic acid are useful in many biotechnological processes especially in food and pharmaceutical areas. The purpose of this study was to evaluate the production of tannase and gallic acid by Aspergillus tamarii developed in submerged and solid state cultures using tannic acid as substrate. In submerged cultures, maximal tannase activity (20,400 ± 2,900 U/L) was obtained after 2 days of cultivation using 2% tannic acid as substrate. In solid state cultivation using polyurethane foam as inert support, maximal tannase activity was obtained after 4 days of cultivation in 15% tannic acid cultures (25,470 ± 1,600 U/L). In both types of cultures, high accumulation of gallic acid was found in the two day-culture filtrates, 0.36±0.05 and 0.67±0.08 g of gallic acid per g of tannic acid, in submerged and solid state cultures, respectively. The accumulation of gallic acid in the cultures is, however, a transitory phenomenon, considering that the fungus slowly absorbs and metabolizes gallic acid.

Key words: Aspergillus tamari, gallic acid, inert support, solid-state cultures, submerged cultures, tannase.