A 22-bp sequence of the core promoter from the Indica rice sucrose-phosphate synthase gene (sps1) is sufficient to confer basal transcription activity
The 5’ region of the sucrose-phosphate synthase gene (sps1) of the Indica riceconsists of an atypical promoter which lacks TATA box but has a putative initiator sequence overriding a second transcription initiation site. Analysis of the transient expression of truncated versions of the sps1 promoter (from -148 to +21) fused to the uidA reporter gene was performed. The...
Nicotinamide: A cytoprotectant against streptozotocin-induced diabetic damage in Wister rat brains
Nicotinamide is being used in experimental and clinical trials examining the prevention of type-1 diabetes mellitus. However, the precise mechanisms underlying the antidiabetic and neuroprotective effects of nicotinamide require further analysis. Our goals are to evaluate the protective effect and the cellular and molecular mechanisms of nicotinamide against brain damage induced by type-1 diabetes in...
Comparative study on the determination of assay for laccase of Trametes sp.
The aim of this work was to determine the sensitivity among compounds in common use for detecting laccase activity. In this study, three assay procedures to measure laccase activity of Trametes sp. were performed in Kirk’s basal salts mediums of three dyes. In the assay methods, three substrates were employed, which were 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS), ο-dianisidine...