Abstract

The amplification and sequencing of 16S rDNA are useful tools to identify important bacteria that cause diseases in the respiratory tracks of a variety of domestic animals. Bordetella bronchiseptica, one of such bacterium, has demonstrated some changes in its metabolic core, generating a greater need for identification through amplification and sequencing of 16S rDNA. We applied this methodology to eleven nasal and pharyngeal isolates from dogs to detect B. bronchiseptica.  Eight strains were completed from which seven showed 98-99% homology; and three strains were only partially sequenced. All eleven strains sequences were deposited in the GenBank (NCBI). What we found was that the sequencing of B. bronchiseptica 16S rDNA is an excellent tool that certifies conventional methods of identification and avoids environmental challenges.

Key words: Bordetella bronchiseptica, 16S rDNA, canine bacteria.