African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Isolation and characterization of two T4-like bacteriophages against pathogenic Escherichia coli of piglet

Nguyen Xuan Hoa
  • Nguyen Xuan Hoa
  • 1. Department of Microbiology and Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; 2. Faculty of Animal Science and Veterinary Medicine, Hue University of Agriculture and Forestry, 102 Phung Hung St., Hue, Vietnam.
  • Search for this author on:
  • Google Scholar
Fang Tang
  • Fang Tang
  • Department of Microbiology and Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
  • Search for this author on:
  • Google Scholar
Qinqin Bai
  • Qinqin Bai
  • Department of Microbiology and Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
  • Search for this author on:
  • Google Scholar
Wei Zhang
  • Wei Zhang
  • Department of Microbiology and Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
  • Search for this author on:
  • Google Scholar
Chengping Lu*
  • Chengping Lu*
  • Department of Microbiology and Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
  • Search for this author on:
  • Google Scholar


  •  Received: 17 May 2014
  •  Accepted: 22 September 2014
  •  Published: 08 October 2014

Abstract

This study, has described the isolation of two T4-like JH11 and JH14 phages. All bacteriophages produced clear plaques in double Luria Bertani (LB) agar, but JH11 produced the larger plaques. These phages lysed Escherichia coli O141: F18 (ETEC), they have icosahedral heads, necks and contractile tails, and belonged to the Myoviridae family. The genomes of these phages were a strand DNA, predicted to be larger than 2 kb. Titer of the two phages were unaffected by exposure to pH 5 - 9 for 16 h. Among the phages, JH11 and JH14 were resistance to enterotoxigenic E. coli O141: F18 (ETEC). Both phages contain three abundant protein bands with approximate molecular sizes of 80, 75 and 50 kDa for JH11, and 50, 38, and 36 kDa for JH14. According to the blast results, the phage JH11 random clones are in close relation with phage T4 gene 23 for major capsid protein MVSS (98%), ECML-134 (98%), T6(97%) and T4 (96% identity) identity. Phage JH14 random clones are in close relation with HX01 (96%), CEV1 (95%) and T4 (93%) identity. The two strains belong to T4-like phage genus. Gene of JH11 and JH14 were (93%) identical.

Key words: Bacteriophage, enterotoxigenic, Escherichia coli, T4-like phage.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 8 No. 41
Back to articles
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES