African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5230

Full Length Research Paper

Antibacterial activity of methanol and ethanol leaf extracts of Antidesma venosum and Lannea barteri

  Adegoke, S. A.*, Agada, F. D. and Ogundipe, L. O.
Department of Microbiology, Faculty of Natural Sciences, Kogi State University, Anyigba, Kogi State, Nigeria.
Email: [email protected]

  •  Accepted: 19 November 2010
  •  Published: 01 July 2013

Abstract

 

Methanol and ethanol cold percolation leaf extracts of  Antidesma venosum and Lanneabarteri were screened for their in vitro antibacterial activity against Staphylococcus aureusEscherichia coliProteus vulgarisSalmonella typhiStreptococcus lactis and Shigella sp. using the agar well diffusion method. Methanol extracts gave higher filtrate of 16.57 and 9.44 g for A. venosum and L. barteri, respectively, while ethanol extract gave 13.52 and 7.10 g, respectively, from 500 g ground leaves extracted with 500 ml of each solvent. Both solvent extracts of A. venosum showed activity against all the clinical test organisms, with zone of inhibition range of 11 to 21 mm diameter except against S. aureus. S. lactis and Shigella sp. were the least susceptible to both solvent extracts of L. barteri. The minimum bactericidal concentration (MBC) range for A. venosum methanol leaf extracts against the clinical test organisms was 6.25 to 50 mg/ml, while for ethanol leaf extract, MBC range was 6.25 to 12.5 mg/ml. MBC range for L. barteri methanol and ethanol leaf extracts were 6.25 to 50 mg/ml and 6.25 to 12.5 mg/ml, respectively. The MBC killing rate of Avenosum methanol leaf extract shows that S. typhi was the most rapidly killed at a rate of 1.88 x 106 CFU/min, while S. aureus was the most rapidly killed by L. barteri methanol leaf extract MBC at a rate of 1.45 x 106 CFU/min.

 

Key words: Antibacterial, extract, inhibitory, bactericidal, susceptibility.