African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Antifungal and antiaflatoxigenic activities of some plant extracts

Al-Rahmah N.1, Mostafa A.1, and Abdel-Megeed A.1,2
  1Department of Botany and Microbiology, Collage of Science, King Saud University, P. O. 2455, Riyadh, 11451 Kingdom of Saudi Arabia. 2Faculty of Agriculture (Saba Basha), Alexandria University, Egypt.
Email: [email protected], [email protected]

  •  Accepted: 10 May 2011
  •  Published: 04 June 2011

Abstract

 

The efficiency of five methanolic plant extracts obtained from Euclayptus globolus, Olea europaeaSalvadora persica, Thymus vulgaris and Ziziphus spina-christi were investigated for their inhibitory effect on the growth and aflatoxin B1 production of the toxigenic Aspergillus flavus strain isolated from wheat seeds. Extract of T. vulgarisshowed the highest antifungal and antiaflatoxigenic activities as aflatoxin B1 production was absolutely arrested at 5 mg/ml, while the mycelial growth of A. flavus was completely inhibited at 15 mg/ml. E. globolus and O. europaea extracts showed a moderate antifungal activities and exhibited a significant antiaflatoxigenic efficiency as they inhibited aflatoxin B1production at 15 mg/ml. On the other hand, S. persica showed weak antifungal activities while no effect was detected with Z. spina-christi extractThe analysis of plant extracts by GC/MS revealed that T. vulgaris extract was mainly composed by thymol (38.73%), carvacrol (19.31%) and ρ-cimene (10.13%), O. europaea extract was composed by anthracenedione (20.63%), terbutaline (13.96%) and propiolic acid (12.02%) while eucalyptol (30.62%), globulol (18.94%) and silane (9.13%) were the principal components of E. globolus extract. These extracts of T. vulgarisE. globolus and O. europaea which are effective and environmentally safe are promising for protecting wheat seeds against aflatoxigenic strain of A. flavus.

 

Key words: Aflatoxins, wheat, antiaflatoxigenic, methanolic extracts, Euclayptus globolus, Olea europaea, Salvadora persica, Thymus vulgaris.