African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5241

Full Length Research Paper

Assessing Listeria monocytogenes hly A genome in pregnant women with spontaneous abortion using PCR method in Yasuj, south west of Iran

  Jahangirsisakht, A.1*, Kargar, M.2, Mirzaee, A.3, Akbartabar Toori M.4, Aramesh S. H.1, Mohamadkhani, N.5 and Dosti, A.6
  1Cellular and Molecular Research Center, Yasuj University of Medical Science, Yasuj, Iran. 2Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran. 3Medicinal Plants Research Center, Yasuj University of Medical Science, Yasuj, Iran. 4Social Determinant of Health Research Center, Yasuj University of Medical Science, Yasuj, Iran. 5Research Center of Education, Yasuj, Iran. 6Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Email: [email protected]

  •  Accepted: 10 May 2013
  •  Published: 16 August 2013

Abstract

 

Listeriosis is a disease which is caused by Listeria monocytogenes bacterium that endangers lives of infants, pregnant women, elderly and patients with suppressed immunity system. The aim of this study was to assess the prevalence of L. monocytogenes in pregnant women with spontaneously abortion and isolating it using polymerase chain reaction method in Yasuj. In a cross-sectional study, 311 specimens were collected from 107 pregnant women including urine, blood, placenta and cervix swab. Among the specimens, 190 samples were from pregnant women with history of abortion and 120 samples from the cases with normal delivery. The specimens were enriched for a period of 4 weeks at 4°C. The samples were studied for hly A genome using polymerase chain reaction method. Among 107 pregnant women, in 11 cases (10.28%), L. monocytogenes hly A gene was detected, with the highest frequency in the age group of 26-30 years old. The mean age of participants was 26.7 years and almost 64% of the positive cases belonged to the cervix. The results of this study show that pregnant women should be checked for L. monocytogenes. Also, further study on newborns with meningitis for this bacterium is recommended in the region.

 

Key words: Listeria monocytogenes, abortion, pregnant women, hly A.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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