The dye methyl red was completely decolorized at 24 h of incubation by Pseudomonas putida MR1 isolated from the dyestuff contaminated soil, collected from the textile industrial area of Sanganer, Jaipur, Rajasthan. Maximum decolorization was achieved when the isolate was incubated at 34°C and pH 7. The decolorization was confirmed by studying the spectral analysis of the dye. 16S rRNA partial gene sequencing (772 bp) of isolate P. putida MR1 was also performed and a phylogenetic tree was prepared.
Key words: Pseudomonas putida MR1, methyl red, decolorization
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