African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Molecular characterization of Trichoderma longibrachiatum 21PP isolated from rhizospheric soil based on universal ITS primers

Mohammad Shahid1*, Mukesh Srivastava1, Antima Sharma1, Anuradha Singh1, Sonika Pandey1, Vipul Kumar1, Neelam Pathak2 and Smita Rastogi2
1Biocontrol Laboratory, Department of Plant Pathology, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur-208002, Uttar Pradesh, India. 2Department of Biosciences, Integral University, Lucknow 226026, Uttar Pradesh, India.
Email: [email protected]

  •  Accepted: 20 September 2013
  •  Published: 11 October 2013


Trichoderma species are morphologically very similar and were considered for many years as a single species. A new strain Trichoderma longibrachiatum 21PP was isolated from a rhizospheric soil sample collected from Kaushambi district of an Indian State (Uttar Pradesh). The universal primers (ITS-1& ITS-4) were used for the amplification of 28S rRNA gene fragment that produced a sharp band of about 700 bp on the gel. The amplified gene fragment was then sequenced (664 bp) and then deposited in GenBank with the Accession No. JX978542. Thus, the molecular identification of the specified strain enabled us for further characterization as this strain of Trichoderma species can be used as a biocontrol agent against pathogenic fungi such as Fusarium, Alternaria, Sclerotinia, etc. It is proposed that the identified strain T. longibrachiatum 21PP be assigned as the type strain of a species of the genus together with the 28S rRNA gene sequence search in Ribosomal Database Project, small subunit rRNA and large subunit rRNA databases.


Key words: 28S ribosomal RNA gene, Trichoderma, polymerase chain reaction, phylogenetic analysis, DNA isolation, DNA sequencing.