African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5230

Short Communication

Mutations in the quinolone resistance determining region in isogenic mutant Acinetobacter baumannii strains

  Liu Hongbo1, Sunwen Zhen2, Xu Xiaoman1 and Li Shengqi1*
  1Department of Respiratory Medicine, Shengjing Hospital, China Medical University, Shenyang 110004, P. R. China. 2Department of General Surgery, Shenyang Fifth People Hospital, Shenyang, Liaoning 110023, P. R. China.
Email: [email protected]

  •  Published: 05 July 2013

Abstract

 

The aim of the study is to explore the change or mutation of DNA gyrase (gyrA) and topoisomerase IV (parC) in Quinolone Resistance Determining Region (QRDR) inAcinetobacter baumannii isogenic mutant strains which were insensitivity or resistant to fluoroquinolones (FQs). A total of 11 strains were studied, one is isolated from clinical sample as a wide-type parent strain and the others isogenic mutant strains which were selected by ciprofloxacin in vitro environment. Three of the isogenic mutant strains have amino acid mutation in GyrA and ParC of QRDR, two of the three have the mutation Ser-97 to Thr and Asp-150 to His in GyrA, another one which is highly resistant to ciprofloxacin (MICs of ciprofloxacin, 64 μg/ml) has the mutations Ser-97 to Thr in GyrA and Ala-85 to Pro in ParC.

 

Key words: Acinetobacter baumannii, quinolone resistance determining region, mutation, isogenic strain.