African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Surface display of multi-epitopes synthetic gene of Mycobacterium tuberculosis on Salmonella typhi Ty21a using a pgsA anchor protein

Badrul S. M. Zainuddin1*, Mustaffa Musa2 and Zainul F. Zainuddin3
  1Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. 2School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian Kelantan, Malaysia. 3School of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Email: [email protected]

  •  Accepted: 28 March 2011
  •  Published: 18 May 2011

Abstract

 

We have developed a recombinant Salmonella typhi Ty21a vaccine candidate (rSTvacIII) displaying a multi-epitopes mycobacterial antigen called the VacIII (containing ubiquitin and 4 immunogenic mycobacterial epitopes of ESAT-6, PhoS1, Hsp 16.3 and MTB 8.4 genes) on the surface of S. typhi Ty21a (Ty21a) using PgsA protein as a carrier. The expression of VacIII protein on the surface of Ty21a was verified by western blot. Immunization in BALB/c mice with rSTvacIII induced strong Th1-type responses based on high level of in vitro IFN-γ but low IL-4 secretion. Since the protective immunity against tuberculosis is mediated by Th1-type response, the rSTvacIII have potential as effective vaccine candidate against tuberculosis.

 

Key words: VacIII antigen, PgsA anchoring motif, tuberculosis, Salmonella typhi Ty21a.