We have developed a recombinant Salmonella typhi Ty21a vaccine candidate (rSTvacIII) displaying a multi-epitopes mycobacterial antigen called the VacIII (containing ubiquitin and 4 immunogenic mycobacterial epitopes of ESAT-6, PhoS1, Hsp 16.3 and MTB 8.4 genes) on the surface of S. typhi Ty21a (Ty21a) using PgsA protein as a carrier. The expression of VacIII protein on the surface of Ty21a was verified by western blot. Immunization in BALB/c mice with rSTvacIII induced strong Th1-type responses based on high level of in vitro IFN-γ but low IL-4 secretion. Since the protective immunity against tuberculosis is mediated by Th1-type response, the rSTvacIII have potential as effective vaccine candidate against tuberculosis.
Key words: VacIII antigen, PgsA anchoring motif, tuberculosis, Salmonella typhi Ty21a.
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