African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Prevalence of Helicobacer pylori in saliva and dental plaque related to periodontal disease and gastritis

Myriam A. De La Garza-Ramos1,2, Jesús A. Valdez-Gonzalez1,2, Rogelio A. Elizondo-Pereo3, Benito Pereyra-Alférez4, Raúl G. Caffesse5, Francisco González-Salazar3,6
1Facultad de Odontología, Universiad Autónoma De Nuevo León, Monterrey, México. 2Centro de Investigaciones y Desarrollo en Ciencias de la Salud, Universidad Autónoma de Nuevo Leon, Monterrey, México. 3División de Ciencias de la Salud, Universidad de Monterrey, Monterrey, México. 4Departamento de Biología Celular, Facultad de Biología Universidad Autónoma de Nuevo León, Monterrey, México. 5University of Texas Health Science Center at Houston, Houston, USA. 6División de Biología Celular y Molecular, Centro de Investigaciones Biomédicas del Noreste, Instituto Mexicano del Seguro Social. Monterrey, México.
Email: [email protected]

  •  Accepted: 10 May 2013
  •  Published: 21 May 2013


Helicobacter pylori is a major etiologic factor in the development of gastritis and peptic ulcer disease. Controversially, the oral cavity has been proposed as a reservoir for this bacterium. The aim of the present study was to determine the prevalence of H. pylori in saliva and dental plaque of adult patients affected by periodontal disease, with or without gastrointestinal symptoms, using polymerase chain reaction (PCR). Suitable PCR primers were designed using sequences of H. pylori DNA from the GenBank database. Chronic periodontal disease was clinically and radiologically diagnosed. The samples for PCR were obtained from periodontal pockets. Cell lysate was centrifuged and used as a DNA source for the PCR assays. The PCR products were analyzed in agarose gels and the sizes of the amplicons were estimated by comparison with DNA size markers. Two samples from each patient were obtained, one from dental plaque and the other from saliva. All salivary samples were negative to PCR isolation of H. pylori, while 13.3% of the plaque samples were positive. All patients with positive PCR isolation of H. pylori had been showing gastrointestinal symptoms. PCR isolation of H. pylori was directly related to the depth of the periodontal pocket.


Key words: Periodontitis, gastritis, polymerase chain reaction, Helicobacter pylori.