African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

In vitro antagonistic efficacy of plant extracts on the enzyme activity of Colletotrichum gloeosporioides

Rangarajulu Senthil Kumaran1*, Yong-Keun Choi1, Hyeon Jin Jeon1, Heehoon Jung1, Kyung-Guen Song2 and Hyung Joo Kim1*
1Department of Biological Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-Gu, Seoul 143-701, Republic of Korea. 2Water Research Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul 130-650, Republic of Korea
Email: [email protected], [email protected]

  •  Accepted: 15 March 2013
  •  Published: 19 March 2013


Anthracnose fungus, Colletotrichum gloeosporioides was isolated in culture from the diseased fruit samples of chilli (Capsicum annuum). The fungus was identified by morphological and molecular analysis. Plant root extracts of A. precatorius and R. tetraphylla and chemical fungicide, dithane-M45 were tested for their inhibitory effects on the cell wall degrading enzymes of C. gloeosporioides under in vitro condition. The activity of cell wall degrading enzymes viz. polymethyl galacturonase (PMG), pectin transeliminase (PTE) and the carboxymethyl cellulase (CMC) was assayed. Root extracts of A. precatorius was found to be more efficient in inhibiting both the pectinolytic (PMG and PTE) and cellulolytic (CMC) enzymes than dithane-M45. The inhibitory effects of enzyme might be due to the presence of fungitoxic compounds such as phenolics, proteins and flavonoids in the root extracts of A. precatorius. On the contrary, extract of R. tetraphyllawas found to induce enzyme activity.


Key words: Plant extract, botanical pesticide, pectinolytic enzyme, cellulolytic enzyme.