African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Optimization of bioprocess for enhanced production of alkaline protease by a Bacillus subtilis SHmIIIa through Plackett-Burman design

S. Jeevan Chandra*
  • S. Jeevan Chandra*
  • Department of Microbiology, Kakatiya Government College, Warangal-506001, T. S, India
  • Search for this author on:
  • Google Scholar
P. Pallavi
  • P. Pallavi
  • Department of Microbiology, Government Degree College for Women, Nalgonda-508001, T. S, India
  • Search for this author on:
  • Google Scholar
B. S. Anuradha
  • B. S. Anuradha
  • Department of Microbiology, Chaitanya P. G. College, Warangal-506001, T. S, India
  • Search for this author on:
  • Google Scholar
S. Ram Reddy
  • S. Ram Reddy
  • Department of Microbiology, Kakatiya University, Warangal- 506009, T. S, India
  • Search for this author on:
  • Google Scholar


  •  Received: 19 November 2014
  •  Accepted: 10 July 2015
  •  Published: 15 July 2015

Abstract

Optimal conditions for the maximum production of alkaline protease by Bacillus subtilis SHmIIIa were evaluated by Plackett-Burman design. Nine process parameters namely, pH, temperature, agitation, inoculum, glucose, peptone, KH2PO4, FeSO4 and tween 20 at two levels were selected for the design. Out of nine selected parameters, six parameters (pH 9.75, agitation 225 rpm, inoculum 3.5%, glucose 5.5 g/L, peptone 3.5 g/L and KH2PO4 1.5%, have shown significant influence on alkaline protease production. Optimization has resulted in spectacular enhancement in alkaline protease yield from 966 to 36000 EU/mg/ml with 37.26 fold increase.

Key words: B. subtilisSHmIIIa, alkaline protease, optimization, design of experiments, Plackett-Burman Design.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 9 No. 28
Back to articles
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES