African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5231

Full Length Research Paper

Characterization of CTX-M type extended spectrum β-lactamases of Escherichia coli isolated from urinary tract infections in Southern Turkey: The first report of CTX-M-14 producing Escherichia coli from Turkey

Mumtaz GURAN1*, Beril AKCIMEN1, Tulin GOKMEN1, Akgün YAMAN2 and Fatih KOKSAL1
1Department of Microbiology, Faculty of Medicine, University of Cukurova, Adana, Turkey. 2Central Labaratory of Balcali Hospital, University of Cukurova, Adana, Turkey.  
Email: [email protected]

  •  Accepted: 08 February 2013
  •  Published: 12 February 2013

Abstract

The aim of this study was to investigate the frequency of CTX-M type extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains which have become emerging etiologic agents of urinary tract infections (UTIs). A total of 310 E. coli isolates recovered from UTIs were screened for ESBL production by VITEK2 system and the double-disk synergy tests. In addition, ESBL positive isolates were selected for typing of blaCTX-Mgenes by PCR screening followed by DNA sequencing analysis permitting the differentiation of the CTX-M subtypes. A pulsed-field gel electrophoresis (PFGE) assay was also performed to check for a possible clonal relation among these isolates. ESBL positivity was detected in 77 (24.8%) of isolates. Subsequently, 46 (14.8%) of ESBL positive E. coli isolates were shown to be carrying blaCTX-M genes of which 30 (9.6%) were found to be CTX-M-15 producers, while the remaining 16 (5.1%) were CTX-M-14 producers. No major clonal relationship among these blaCTX-M producers was found. Wedemonstrated the presence of CTX-M-14 producer E. coli strains for the first time in Turkey and provided epidemiologic data suggesting the evidence for the ongoing dissemination of CTX-M type ESBLs.

 

Key words: CTX-M-14, extended-spectrum beta-lactamase (ESBL), Escherichia coli, pulsed-field gel electrophoresis (PFGE)