African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Analysis of biochemical divergence for classification of six native silkworm based on total cholesterol, triglycerides, high density lipoproteins (HDL) cholesterol, low density lipoproteins (LDL) cholesterol, total lipase and lipids

Alireza Seidavi
Department of Animal Science, Rasht Branch, Islamic Azad University, Rasht, Iran.
Email: [email protected]

  •  Accepted: 30 July 2011
  •  Published: 23 September 2011


This research studies a comparison between the levels and the abundance total cholesterol, triglycerides, HDL cholesterol (High Density Lipoproteins), LDL cholesterol (Low Density Lipoproteins), total lipase and lipids in six native groups of silkworm. These six biochemical parameters and enzymes play important roles in the silk production, digestion and other physiological activities. Other aim of this experiment was study and classification of these six native silkworm based on biochemical parameters. In 5th day of 5th instar, heamolymph sampled using standard method. Sampled heamolymph transferred to laboratory. Heamolymph was obtained by cutting abdominal proleg and collected into 1.5 ml tube containing a few granules of phenylthiourea to prevent melanization. After 10 min centrifugation at 10000 rpm, the supernatant was used. Pellets was discarded also. The supernatant was transferred to new tubes and was preserved at -20ºC until the onset of the experiments. Hierarchical agglomerative clustering was done by using NTSYS-pc, based on Un weighted pair-group method using arithmetic average (UPGMA). From obtained results, it is showed that amount of total lipase in six studied local varieties included between 79.26 to 176.08 IU/L. Among studied local varieties, the highest level of total lipase belonged to Pink Khorasan (176.08 IU/L), and Lemon Haratee and Baghdadi variety (79.26 IU/L) remained at lower level than other varieties. Other varieties were between these two groups. Meanwhile statistical differences between studied varieties for this trait were not significant (P>0.05). Analyzed varieties were divided into three distinct groups. At cross 2.53, two clusters were formed which classified into subgroups in crosses of 0.80. Frequent divisions were also observed in major groups. First group includes Baghdadi variety and second group includes Pink Khorasan variety.


Key words: Silkworm, triglyceride, hierarchical agglomerative cluster, biochemical marker.