Genotypic characterization of Escherichia coli strains isolated from dairy cattle environment

Greiciane Franca Bronzato; Naiara Miranda Bento Rodrigues; Bruno Rocha Pribul; Gabrielli Stefaninni Santiago; Irene da Silva Coelho; Miliane Moreira Soares de Souza; Elina Reinoso; Mirta Lasagno; Shana de Mattos de Oliveira Coelho

doi:10.5897/AJMR2017.8677

African Journal of
Microbiology Research

Abbreviation: Afr. J. Microbiol. Res.
Language: English
ISSN: 1996-0808
DOI: 10.5897/AJMR
Start Year: 2007
Published Articles: 5233

Full Length Research Paper

Genotypic characterization of Escherichia coli strains isolated from dairy cattle environment

Greiciane Franca Bronzato

Greiciane Franca Bronzato
Veterinary Bacteriology Laboratory, Federal Rural University of Rio de Janeiro, UFRRJ, Rio de Janeiro, Brazil.
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Naiara Miranda Bento Rodrigues

Naiara Miranda Bento Rodrigues
Veterinary Bacteriology Laboratory, Federal Rural University of Rio de Janeiro, UFRRJ, Rio de Janeiro, Brazil.
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Bruno Rocha Pribul

Bruno Rocha Pribul
National Reference Laboratory for Enteric Diseases, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.
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Gabrielli Stefaninni Santiago

Gabrielli Stefaninni Santiago
Veterinary Bacteriology Laboratory, Federal Rural University of Rio de Janeiro, UFRRJ, Rio de Janeiro, Brazil.
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Irene da Silva Coelho

Irene da Silva Coelho
Veterinary Bacteriology Laboratory, Federal Rural University of Rio de Janeiro, UFRRJ, Rio de Janeiro, Brazil.
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Miliane Moreira Soares de Souza

Miliane Moreira Soares de Souza
Veterinary Bacteriology Laboratory, Federal Rural University of Rio de Janeiro, UFRRJ, Rio de Janeiro, Brazil.
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Elina Reinoso

Elina Reinoso
Microbial Genetics Laboratory, National University of Rio Cuarto, UNRC, RÃo Cuarto, Argentina.
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Mirta Lasagno

Mirta Lasagno
Microbial Genetics Laboratory, National University of Rio Cuarto, UNRC, RÃo Cuarto, Argentina.
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Shana de Mattos de Oliveira Coelho

Shana de Mattos de Oliveira Coelho
Veterinary Bacteriology Laboratory, Federal Rural University of Rio de Janeiro, UFRRJ, Rio de Janeiro, Brazil.
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Article Number - 57A72DE55259
Vol.11(47), pp. 1669-1675 , December 2017
https://doi.org/10.5897/AJMR2017.8677

Received: 13 August 2017
Accepted: 15 November 2017
Published: 21 December 2017

Abstract

The aim of this study was to characterize the diversity of Escherichia coli strains involved in the dispersion of virulence genes. 152 E. coli strains originated from dairy cattle environment were evaluated through phenotypic and proteomic assays. These samples were investigated for the presence of virulence genes (eaeA, stxI, stxII, ST, LT, eagg, ial) and biofilm related genes (fimH, csgA, flu). Eighteen profiles were obtained and 30 isolates were selected for macrorestriction assay by Pulsed-Field Gel Electrophoresis (PFGE) technique. PFGE patterns of XbaI-digested performed to determine the clonal relatedness of E coli isolates. A total of 27 pulsotypes of E. coli were identified with a low percentage of genetic similarity (≤68%) demonstrating a high genetic diversity in the isolates tested. Furthermore, the presence of biofilm-associated related gene (fimH) can contribute to the dispersion and persistence of this pathogen into the milk environment. Also, all of the pulsotypes (n = 8) with genetic similarity from 95% were present in milk samples, feces and liner were found in different seasons of the year, sharing at least one gene associated with biofilm formation. So the characterization of the genetic diversity of E. coli is important for understanding its dispersion in order to assist in the implementation of measures to prevent the spread of these agents in milk production environment.