African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Effect of Helicobacter pylori infection on oxidative stresses in patients with chronic gastritis

Azita Navvabi1, Mohammad Hassan Khadem Ansari2*, Nazila Navvabi3, Sara Khadem Ansari4 and Yousef Rasmi2
1Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran.  2Department of Clinical Biochemistry, Urmia Medical School, Urmia, Iran. 3Department of Veterinary Medicine, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. 4Department of Medicine Tabriz Azad University, Tabriz, Iran.
Email: [email protected]

  •  Accepted: 06 November 2013
  •  Published: 31 December 2013

Abstract

The Helicobacter pylori infection is currently endemic worldwide with high prevalence in developing regions. The infection causes chronic gastritis, gastric and gastric adenoid carcinoma. Infection by H. pylori may act as a risk factor for proteins, lipids and DNA damages. In this study, gastric biopsies were obtained in patients with a chronic gastric to investigate effects of H. pylori infection on oxidative stress in patients with chronic gastritis. Urease test and polymerase chain reaction (PCR) were performed on endoscopic gastric biopsy obtained from 150 subjects. The result showed that the presence of a 492-bp DNA fragment was indicative of a positive reaction, also fresh samples had a positive urease test for H. pylori. Malondialdehyde (MDA) in patients with gastritis as a result of H. pylori were significantly increased as compared to the control groups (p<0.05). Mean ± SE of reduced glutathione (GSH) and total antioxidant capacity (TAC) in patients were decreased significantly (p<0.05). The result shows mean ± SE of oxidized glutathione (GSSG) in patients showed significant increased as compared to the control groups. These finding show that H. pylori infection can cause oxidative stress disorders that may act as risk factors.

 

Key words: Malondialdehyde, reduced glutathione, oxidized glutathione, total antioxidant capacity, Helicobacter pylori.