African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5230

Full Length Research Paper

Investigation of Entamoeba histolytica and Mycobacterium spp. in biopsy specimens of patients with inflammatory bowel disease in Turkey

  Gülay Aral Akarsu1*, Zeynep Ceren Karahan2, R. Engin Araz3, ÇiÄŸdem Güngör1 and Mehmet Tanyüksel3
  1Department of Medical Parasitology, Ankara University Medical Faculty, Dekanlik Binasi 06100 Sihhiye, Ankara, Turkey. 2Department of Medical Microbiology, Ankara University Medical Faculty, Dekanlik Binasi, 06100 Sihhiye, Ankara, Turkey. 3Division of  Medical Parasitology, Gülhane Military Medical Academy, General Doktor Tevfik SaÄŸlam Caddesi 06018 Etlik, Ankara, Turkey.
Email: [email protected]

  •  Accepted: 30 March 2011
  •  Published: 18 April 2011



Intestinal amebiasis and gastrointestinal tuberculosis can mimic inflammatory bowel disease and its exacerbations clinically, pathologically, radiologically and endoscopically. In the existence of IBD and/or either one of these two pathogens, early identification and prompt treatment can improve the clinical course of the patients. The aim of this study was to investigate the presence of Entamoeba histolytica and/or Mycobacterium spp. in the first diagnostic biopsy specimens of prediagnosed IBD patients in a tertiary education hospital in Ankara, Turkey. As the differentiation of pathologic Entamoeba histolytica must be based on isoenzymatic, immunologic or molecular analysis and PCR is a rapid and reliable method for the identification of  Mycobacterium spp., we investigated the presence of these pathogens in the biopsy specimens of 20 patients who were suspected to have IBD and nine controls, by using PCR-based detection methods. All of them were histopathologically diagnosed as Crohn’s disease and none of the specimens contained these two pathogens. We thought that the low prevalence of both infections in Crohn’s disease patients may have caused our negative findings and loss of pathogens could have lowered the sensitivity. Further studies with larger number of patients are needed to determine the misdiagnosis rate and coexistence of these three diseases.


Key words: Entamoeba histolyticaMycobacterium spp., inflammatory bowel disease, Crohn’s disease, olymerase chain reaction (PCR), biopsy.