African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Differential diagnosis of Entamoeba spp. in gastrointestinal disorder patients in Khorramabad, Iran

Farnaz Kheirandish1*, Ebrahim Badparva1, Ali Haghighi2, Ehsan Nazemalhosseini-Mojarad3 and Bahram Kazemi2,4
1Department of Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. 2Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University, M.C., Tehran, Iran. 3Department of Foodborne and Diarrhea Disease, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University, M.C., Tehran, Iran. 4Cellular and Molecular Biology Research Center, Shahid Beheshti University, M.C., Tehran, Iran.
Email: [email protected]

  •  Accepted: 23 June 2011
  •  Published: 16 September 2011

Abstract

Differential diagnosis of Entamoeba spp. has great clinical and epidemiological importance. Entamoeba moshkovskii cysts and trophozoites are morphologically indistinguishable fromEntamoeba dispar and Entamoeba histolytica. This study was carried out for the first time to detect Entamoeba spp. in stool samples by using molecular method from April 2010 to December 2010 in Khorramabad, Iran. A total of 862 fecal specimens were collected from patients having abnormal gastrointestinal symptoms and who were referred to the health care centers of Khorramabad. Out of 862 stool samples, 16 (1.86 %) showed the presence of E. histolytica/E. dispar/E. moshkovskii cysts by microscopic examination. Consequently, single-round PCR was carried out to differentiate the Entamoeba spp. OF the sixteen samples that were microscopically positive, 1 (6.25%) was E. moshkovskii, and 15 (93.75%) were E. dispar. Our results, along with those of other similar study conducted in different parts of Iran, reveal that E. dispar is more prevalent.

 

Key words: Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, single-round PCR, Iran.