Thirteen polymorphic microsatellite loci originating from the genome sequence were developed for cotton pathogenic fungus, Verticillium dahliae. The simple sequence repeats (SSR) markers were used to characterize the genotypic diversity of 30 isolates ofV. dahliae. Thirteen loci displayed the number of alleles per locus (Na) ranged from 2 to 5 with an average of 3 and effective number of alleles (“NE”) with a broader range (1.80 to 3.91). Unbiased expected heterozygosity (“HE”) showed moderate to high levels of variation (0.43 to 0.74). Thirteen primer pairs rendered provide a new scope for population genetic analysis of V. dahliae on cotton.
Key words: Microsatellites, Verticillium dahliae, genotypic diversity, cotton Verticillium wilt.
Abbreviations: RAPD, Random amplified polymorphic DNA; AFLP, amplified fragment length polymorphic; SSR, simple sequence repeat; PCR, polymerase chain reaction; Na, number of alleles; NE, effective number of alleles; HO, observed heterozygosities; HE, expected heterozygosities; HWE, Hardy–Weinberg equilibrium; LD, linkage disequilibrium;NE, effective number of alleles.
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