African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Short Communication

Development of polymorphic microsatellite markers from Verticillium dahliae, a pathogenic fungus of cotton

  Li Zhi-Fang, Zhu He-Qin*, Feng Zi-Li, Zhao Li-Hong, Shi Yong-Qiang, and Yin Zhi-Xin
State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, 455000, China.
Email: [email protected]

  •  Accepted: 10 May 2013
  •  Published: 05 July 2013

Abstract

 

Thirteen polymorphic microsatellite loci originating from the genome sequence were developed for cotton pathogenic fungus, Verticillium dahliae. The simple sequence repeats (SSR) markers were used to characterize the genotypic diversity of 30 isolates ofV. dahliae. Thirteen loci displayed the number of alleles per locus (Na) ranged from 2 to 5 with an average of 3 and effective number of alleles (“NE”) with a broader range (1.80 to 3.91). Unbiased expected heterozygosity (“HE”) showed moderate to high levels of variation (0.43 to 0.74). Thirteen primer pairs rendered provide a new scope for population genetic analysis of V. dahliae on cotton.

 

Key words: Microsatellites, Verticillium dahliae, genotypic diversity, cotton Verticillium wilt.

Abbreviation

AbbreviationsRAPD, Random amplified polymorphic DNAAFLP, amplified fragment length polymorphic; SSR, simple sequence repeat; PCR, polymerase chain reaction; Na, number of alleles; NE, effective number of alleles; HO, observed heterozygosities; HE, expected heterozygosities; HWE, Hardy–Weinberg equilibrium; LD, linkage disequilibrium;NE, effective number of alleles.