African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Induction of non protein thiols by chromium in cyanobacteria isolated from polluted areas

Abdul Hameed1*, Masaya Satoh2, Yuka Furuhashi2, Yasuo Oyama2 and Shahida Hasnain1
1Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore-54590, Pakistan. 2Faculty of Integrated Arts and Sciences. The University of Tokushima, Tokushima 770-8502, Japan.
Email: [email protected]

  •  Accepted: 08 September 2013
  •  Published: 04 October 2013


Two cyanobacterial strains Synechocystis AHZ-HB-MK” and “AHZ-HB-P2A” were isolated from tannery effluent form Lahore, Pakistan and their tolerance against K2CrO4was examined. Although growth was completely inhibited at 10 µg mL-1 in AHZ-HB-P2Astrain, cell growth of AHZ-HB-MK strain was observed even at a concentration of 100 µg mL-1. To show reasons for different tolerance between the two strains, we compared changes in nonprotein thiols, glutathione (GSH and GSSG), cysteine and cystine, after K2CrO4 treatment in both strains. After K2CrO4 treatment GSH completely disappeared in both strains, suggesting that GSH was involved in detoxification. GSSG content in former strain remarkably increased almost three and six times after 7-days treatment with 10 and 100 µg mL-1 K2CrO4, respectively while it decreased in the later strain. Cysteine content in the former strain increased almost three and eleven times after 1 day and 7 days treatment with 100 µg mL-1 K2CrO4, respectively, such increase in cysteine content was not observed in later strain. These data revealed that former strain could induce glutathione and cysteine but later strain could not. The difference in the ability to induce the nonprotein thiols between two strains must be responsible for differential tolerance in K2CrO4 observed in the growth inhibition.


Key words: Chromium, cysteine, cyanobacteria, glutathione, nonprotein thiol,Synechocystis.


GSH, Glutathione reduced form; GSSG, glutathione oxidized form.