African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Real time polymerase chain reaction for identification of Candida species isolated from blood stream infections in non-neutropenic cancer patients

  Rasha A. Alm El-Din*, Hanan El-Bassat, Mohamed El-Bedewy and Mohamed A. Alm El-Din
Medical Microbiology and Immunology, Tropical Medicine, Internal Medicine, Clinical Oncology Departments, Faculty of Medicine, Tanta University, Egypt.
Email: [email protected]

  •  Accepted: 26 August 2013
  •  Published: 27 September 2013



This work aimed at identifying Candida species isolated from blood stream infection in non-neutropenic cancer patients by conventional and molecular methods. Out of 200 blood samples from cancer patients with blood stream infection, 89 were positive forCandida which were identified using chromogenic medium (CHROMagar Candida), and real-time polymerase chain reaction (RT-PCR) assays targeting the fungal ITS2 region using a LightCycler instrument. The present study revealed that non-albicans Candidaspecies caused most of the cases of candidaemia in the cancer patients. A total of 89Candida isolates were obtained, Candida albicans represent 15/89 patients (16.85%) and non-albicans Candida species accounts for 74/89 patients (83.1 %) of which Candida tropicalis represent 32.6% followed by Candida parapsiliosis 23.6%, and then Candida rugosa (21,4%) and the lowest percentage was in Candida glabrata (5.6%). The results of the study revealed that real time-PCR could detect 81 out of 89 cases of candidaemia with sensitivity of 91.01%, specificity of 95.6%, PPV of 94.18% and NPV of 92.30%. The high prevalence of non-albicans candidaemia should be considered prior to antimicrobical therapy in non-neutropenic cancer patients with candidaemia. Real-time PCR represents a rapid and accurate method for diagnosis of candidaemia.


Key words: Candidaemia, Candida albicans, real-time polymerase chain reaction (RT-PCR).