African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Characterization of microbial community in the selected Polish mineral soils after long term storage

Agnieszka WoliÅ„ska*, Zofia StÄ™pniewska and Agnieszka Kuźniar  
Department of Biochemistry and Environmental Chemistry, Institute of Biotechnology, The John Paul II Catholic University of Lublin, ul. Konstantynów 1 I, 20-708 Lublin, Poland
Email: [email protected]

  •  Accepted: 07 February 2013
  •  Published: 12 February 2013


The effect of 19-years storage period at air-dried condition (4°C) and impact of soils rewetting on microbial presence were studied. The topsoil (0 to 20 cm) of Mollic Gleysol,Eutric CambisolRendzina LeptosolOrthic Podzol and Eutric Fluvisol were used in the experiment. It was found that 10-days of soil incubation at full water capacity conditions and room temperature is enough for soil microbial regeneration. The moisture content was determined for a range of water potential (pF) values: 0; 1.5; 2.2; 2.7 and 3.2, corresponding to available water and representing different water availabilities for microorganisms and plant roots. According to the results, soil moisture content significantly increased (P<0.001) the abundance of the total number of bacteria and most probable number (MPN) of ammonia oxidizing bacteria (AOB). Molecular analysis (16S rRNA) shows the dominance of Betaproteobacteria genera with the main representatives of NitrosomonasNitrosospiraDelftiaComamonas and Pseudomonas, as well as exponent species of Firmicutes genera: Clostridium and Ruminococcus.


Key words: 16S rRNA gene analysis, soil water potential, microorganisms abundance, soil rewetting.