African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5230

Full Length Research Paper

A simplified system without purification for selection of aptamers against Vibrio alginolyticus

Jumin Hao, Jiang Zheng*, Xuemin Tang, Qingpi Yan, Yubao Li and Zhongbao Li
Fisheries College of Jimei University, Key Laboratory of Science and Technology for Aquaculture and Food Safety in Fujian Province University, Xiamen 361021, China.
Email: [email protected]

  •  Accepted: 22 September 2011
  •  Published: 09 October 2011

Abstract

 

We have explored a simplified system without purification for systematic evolution of ligands by exponential enrichment (SELEX) against Vibrio alginolyticus to select single-stranded DNA ligands (aptamers) from a random 82-nt library. The DNA content was quantified by agarose gel electrophoresis and follow-up image analyses with theBandScan 5.0 software. Anti-digoxigenin/HRP system was used to determine the binding activity of aptamers for V. alginolyticus. The results showed that the affinity of aptamers increased gradually with the increase of screening rounds, indicating that the lack of purification did not affect screening results but rather made SELEX screening much more convenient and significantly increased the efficiency.

 

Key words: Aptamers, purification, systematic evolution of ligands by exponential enrichment (SELEX), Vibrio alginolyticus.