African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Morphological, pathogenic and molecular characterization of fungal species associated with mango fruits in Mexico

María Alejandra Istúriz-Zapata
  • María Alejandra Istúriz-Zapata
  • Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional. Carretera Yautepec-Jojutla km. 6, calle CEPROBI, No 8 Col. San Isidro, Yautepec, Morelos, CP 62731 México.
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Silvia Bautista-Baños
  • Silvia Bautista-Baños
  • Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional. Carretera Yautepec-Jojutla km. 6, calle CEPROBI, No 8 Col. San Isidro, Yautepec, Morelos, CP 62731 México.
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José Luis Acosta-Rodríguez
  • José Luis Acosta-Rodríguez
  • Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, Boulevar Juan de Dios Bátiz Paredes, San Joachin, Guasave, Sinaloa, CP 81107 México.
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Mónica Hernández-López
  • Mónica Hernández-López
  • Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional. Carretera Yautepec-Jojutla km. 6, calle CEPROBI, No 8 Col. San Isidro, Yautepec, Morelos, CP 62731 México.
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Laura Leticia Barrera-Necha
  • Laura Leticia Barrera-Necha
  • Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional. Carretera Yautepec-Jojutla km. 6, calle CEPROBI, No 8 Col. San Isidro, Yautepec, Morelos, CP 62731 México.
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  •  Received: 08 June 2023
  •  Accepted: 04 August 2023
  •  Published: 31 August 2023

Abstract

The characterisation of the causal agents of postharvest diseases, as well as their pathogenicity in different mango cultivars of economic importance in Mexico was evaluated. In total, 37 fungi were isolated from diseased mango (Mangifera indica L.) of the cvs Ataulfo, Hayden, Manila, Peach, Haden and creole. For the morphological characterization, the isolates were seeded in potato dextrose agar media and described by 4 variables. For the molecular characterization, DNA extraction was performed using the commercial Kit. For amplification, primers ITS5 and ITS4 were used and phylogenetic tree was built using RaxML. Six species were identified, namely Aspergillus niger, Colletotrichum asianum, Lasiodiplodia theobromae, Neofusicoccum oculatum, Pestalotiopsis mangiferea and Talaromyces variabilis. The most abundant group was the genus Aspergillus, with an appearance frequency of 0.35. Phylogenetic analysis showed that C. asianum and P. mangiferae belong to the families Glomerellaceae and Pestalotiopsidaceae, respectively, whereas A. niger and T. variabilis belong to the family Trichocomaceae. The fungi L. theobromae and N. occulatum belong to the family Botryosphaeriaceae. The pathogenicity of all isolates was demonstrated, except for T. variabilis. In contrast, L. theobromae and N. oculatum, were the most pathogenic isolates in all evaluated cultivars. Susceptibility to each pathogen differed among the cultivars, and Creole was most susceptible to the fungi evaluated.

 

Key words: Cultivars, disease postharvest, Mangifera indica L., phylogenetics analysis.