Abstract

In this study it has been shown that hospital sources of Candida species could cause human infections, via identification of the yeasts isolated from cases and also environmental specimens, staff, visitors and patient’s micro flora. Microscopic direct examination was used for detection of Candida pathogenic forms. Differential cultures and molecular tests including; PCR and RFLP were performed for the identification of Candidaspecies. From the isolated fungi, we obtained Candida 36 (66.6%), Aspergillus 17 (31.4%) and Alternaria species only one case. Candida species isolated from clinical samples included: Candida albicans, Candida tropicalis, Candida glabrata, Candida krusei, Candida guilliermondii and Candida parapsilosis. Among all environmental isolated yeasts; Candida albicans (60%), Candida krusei (17%), Candida glabrata (14.3%), Candida tropicalis(0.7%) and Candida parapsillosis (3%) were identified. We believe that Candidacontamination of environmental surfaces and hand touches can facilitate the occurrence of several Candida infections predominantly in patients with immune compromised system.

Key words:  Hospital, infection, Candida, source.

Abbreviation

 PCR, Polymerase chain reaction; RFLP, restriction fragment length polymorphism.