An actinomycete strain T16-1 demonstrated the maximum poly (L-Lactide)-degrading activity when cultured in basal liquid medium at 50°C. According to 16S rDNA sequence analysis, chemotaxonomic and DNA-DNA hybridization revealed that strain T16-1 belong to the family Thermomonosporaceae, genus Actinomadura. On the basis of phenotypic and phylogenic data, strain T16-1 which is a novel PLA-degrading thermophilic actinomycete was identified as Actinomadura keratinilytica, but the color of its colony on ISP plates, NaCl tolerant and utilization of mannitol, raffinose and arabinose were different. Partial sequence of poly (L-lactide) depolymerase gene from strain T16-1 was demonstrated. The gene consisting of 222 amino acids was related to serine protease from Streptomyces sp. with 43 to 46% identity.
Key words: Actinomadura keratinilytica, cloning, identification, poly (L-lactide).
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