African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Effect of different pH values on growth and sporulation of Estye vermicola

Zhen Wang1,2, Chun Yan Wang2, Yun Bo Wang2, Jian Jie Xue2, Zheng Li2, Jing Jie Li2, Li Guan Gu2 and Chang Keun Sung2*
1Engineering Technology Research Center of Southern Shandong Province for TCM Resource Development, Linyi University, Linyi, 276000, People’s Republic of China. 2Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Daejeon, 305-764, South Korea.
Email: [email protected]

  •  Accepted: 17 June 2013
  •  Published: 30 June 2013

Abstract

Esteya vermicola, an endoparasitic fungus of pinewood nematode, exhibits great potential as a biological agent against pinewood nematodes. In order to improve the growth rate, sporulation and proportion of lunate conidia, the optimization of pH value for E. vermicolawas tested. The diameter and dry mass of E. vermicola in PDA medium were increased greatly at pH 5 after 15 days, while the diameter and dry mass of E. vermicola were inhibited at pH 9 and 10. The sporulation of E. vermicola in PDA or PDB medium was highest at pH 6, 2.0 × 107 and 1.4 × 107 CFU/ml, respectively. The proportion of lunate conidia in PDA medium was highest at pH 4, while the proportion of lunate conidia in PDB medium was highest at pH 7.

 

Key words: pH value, Esteya vermicola, growth, sporulation.