African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Screening for nematicidal activities of Beauveria bassiana and associated fungus using culture filtrate

Di Zhao1, Bin Liu2, Yuanyuan Wang1, Xiaofeng Zhu1, Yuxi Duan1 and Lijie Chen1*  
1Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang, Liaoning, China. 2Liaoyuan Agricultural Academy of Science, Liaoyuan, Jilin, China.
Email: [email protected]

  •  Accepted: 08 March 2013
  •  Published: 12 March 2013

Abstract

Using dilution and tissue block isolation methods, we obtained 105 isolates of Beauveria bassiana and seven isolates of its associated fungus. The filtrate of fermentation fluid of these isolates was tested for nematicidal activities against four targeted nematode species. Results indicate that nine isolates had high nematicidal activities against four targeted nematodes. Studies show that the culture filtrate of different isolates of B. bassiana and its associated fungus had different levels of activities against the same nematode, and the same culture filtrate had selective toxicity against different nematodes.

 

Key words: Beauveria bassiana, associated fungus, fermentation fluid, nematode, bioactivity